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乙型肝炎表面抗原和核心抗原单克隆抗体的制备及其在肝活检中病毒抗原检测中的应用。

Production of monoclonal antibodies to hepatitis B surface and core antigens, and use in the detection of viral antigens in liver biopsies.

作者信息

Tedder R S, Guarascio P, Yao J L, Lord R B, Eddleston A L

出版信息

J Hyg (Lond). 1983 Feb;90(1):135-42. doi: 10.1017/s0022172400063932.

DOI:10.1017/s0022172400063932
PMID:6337208
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2134181/
Abstract

Hybridomas secreting monoclonal antibodies to HBsAg and HBcAg were prepared from immunized mice. An antibody capture radioimmunoassay was used to detect and select appropriate hybrids for propagation and cloning. The advantages of this assay were discussed. The resulting monoclonal antibodies were compared with conventional polyclonal antisera for the detection of virus antigens in liver tissue and found to give excellent results.

摘要

从免疫小鼠中制备出分泌针对乙肝表面抗原（HBsAg）和乙肝核心抗原（HBcAg）的单克隆抗体的杂交瘤。采用抗体捕获放射免疫分析法来检测并选择合适的杂交瘤用于传代培养和克隆。讨论了该分析方法的优点。将所得的单克隆抗体与传统的多克隆抗血清用于检测肝组织中的病毒抗原，并发现其效果极佳。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a07e/2134181/d5b89be781ac/jhyg00022-0144-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a07e/2134181/14d1ac7be592/jhyg00022-0145-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a07e/2134181/d5b89be781ac/jhyg00022-0144-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a07e/2134181/14d1ac7be592/jhyg00022-0145-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a07e/2134181/d5b89be781ac/jhyg00022-0144-a.jpg

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Production of monoclonal antibodies to hepatitis B surface and core antigens, and use in the detection of viral antigens in liver biopsies.乙型肝炎表面抗原和核心抗原单克隆抗体的制备及其在肝活检中病毒抗原检测中的应用。
J Hyg (Lond). 1983 Feb;90(1):135-42. doi: 10.1017/s0022172400063932.
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本文引用的文献

1
Analysis of antigenic drift in the haemagglutinin molecule of influenza B virus with monoclonal antibodies.用单克隆抗体分析乙型流感病毒血凝素分子中的抗原漂移
J Gen Virol. 1981 Jun;54(Pt 2):243-51. doi: 10.1099/0022-1317-54-2-243.
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Use of an enzyme-linked immunosorbent assay for screening hybridoma antibodies against hepatitis B surface antigen.使用酶联免疫吸附测定法筛选抗乙型肝炎表面抗原的杂交瘤抗体。
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The production of monoclonal antibodies to rubella haemagglutinin and their use in antibody-capture assays for rubella-specific IgM.
通过改进的免疫化学方法检测到乙肝感染肝脏中广泛存在细胞质乙肝核心抗原。
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Definition of hepatitis B virus (HBV)-specific target antigens recognized by cytotoxic T cells in acute HBV infection.急性乙型肝炎病毒（HBV）感染中细胞毒性T细胞识别的HBV特异性靶抗原的定义。
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Recognition of several idiotopes on a monoclonal anti-hepatitis B virus surface antigen using monoclonal anti-idiotypic antibodies.使用单克隆抗独特型抗体识别单克隆抗乙型肝炎病毒表面抗原上的多个独特型表位。
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In-vitro cell-mediated cytotoxicity for autologous liver cells in chronic non-A, non-B hepatitis.慢性非甲非乙型肝炎中自体肝细胞的体外细胞介导细胞毒性
Clin Exp Immunol. 1986 Jan;63(1):147-55.
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A surrogate hepatitis B virus antigenic epitope represented by a synthetic peptide and an internal image antiidiotype antibody.一种由合成肽和内影像抗独特型抗体呈现的替代乙型肝炎病毒抗原表位。
J Exp Med. 1986 Jul 1;164(1):227-36. doi: 10.1084/jem.164.1.227.
8
Monoclonal 'internal image' anti-idiotypic antibodies of hepatitis B surface antigen.乙肝表面抗原的单克隆“内影像”抗独特型抗体。
Immunology. 1985 Jun;55(2):197-204.
9
Molecular mimicry of hepatitis B surface antigen by an anti-idiotype-derived synthetic peptide.一种抗独特型衍生合成肽对乙型肝炎表面抗原的分子模拟
Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11900-4. doi: 10.1073/pnas.89.24.11900.
风疹血凝素单克隆抗体的制备及其在风疹特异性 IgM 抗体捕获试验中的应用。
J Hyg (Lond). 1982 Apr;88(2):335-50. doi: 10.1017/s0022172400070182.
4
Contrasting patterns and frequency of antibodies to the surface, core, and e antigens of hepatitis B virus in blood donors and in homosexual patients.献血者和同性恋患者中针对乙型肝炎病毒表面、核心和e抗原的抗体的对比模式及频率
J Med Virol. 1981;6(4):323-32. doi: 10.1002/jmv.1890060407.
5
Australia antigen in liver tissue--an immunofluorescent and immunoelectron microscopic study.肝组织中的澳大利亚抗原——一项免疫荧光和免疫电子显微镜研究
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Continuous cultures of fused cells secreting antibody of predefined specificity.分泌预定义特异性抗体的融合细胞的连续培养物。
Nature. 1975 Aug 7;256(5517):495-7. doi: 10.1038/256495a0.
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Pattern of core and surface expression in liver tissue reflects state of specific immune response in hepatitis B.肝组织中核心和表面表达模式反映了乙型肝炎中特异性免疫反应的状态。
Lab Invest. 1975 Jan;32(1):1-9.
8
Distribution patterns of hepatitis B surface antigen (HBsAg) in the liver of hepatitis patients.乙肝患者肝脏中乙肝表面抗原（HBsAg）的分布模式。
J Clin Pathol. 1976 Feb;29(2):94-100. doi: 10.1136/jcp.29.2.94.
9
Hepatitis B core and surface antigens in liver tissue. Light and electron microscopic localization by the peroxidase-labeled antibody method.肝组织中的乙肝核心抗原和表面抗原。用过氧化物酶标记抗体法进行光镜和电镜定位
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Detection of HBSAG in fixed liver tissue - use of a modified immunofluorescent technique and comparison with histochemical methods.固定肝组织中乙肝表面抗原的检测——改良免疫荧光技术的应用及与组织化学方法的比较
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