Department of Biophysics, Faculty of Medicine, Bolu Abant Izzet Baysal University, 14030, Bolu, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey.
Naunyn Schmiedebergs Arch Pharmacol. 2020 Nov;393(11):2107-2115. doi: 10.1007/s00210-020-01925-y. Epub 2020 Jun 26.
Antiinflammatory properties of pulsed magnetic field (PMF) treatments or administration of antiLy6G antibody have been previously reported. In this study, we hypothesized that, the combination of PMF treatments and antiLy6G administration may synergistically potentiate their antiinflammatory actions. The effects of the combination of PMF treatments and antiLy6G administration were investigated by examining the inflammatory signs, histopathological properties of the inflamed site, and measuring the macrophage inflammatory protein-1 alpha (MIP-1α/CCL3) and myeloperoxidase (MPO) levels of inflamed paw tissues in rats with carrageenan-induced acute paw inflammation. In this present study, PMF treatments alone or administration of antiLy6G alone ameliorated the acute inflammation. However, their combination exacerbated the inflammatory signs, hyperalgesia, allodynia, edema and fever, and aggravated the inflammatory conditions by excessive infiltration of inflammatory cells to the inflamed site. These opposing effects of the combined treatments may correlate with enhanced levels of MIP-1α and MPO in inflamed paws. Present results indicated that the combination of the PMF treatments and antiLy6G administration may not provide additional benefits and may actually cause an aggravation of the acute inflammatory process. Findings may also suggest that during neutrophil or immune cell-targeted treatments for inflammatory states, magnetic field exposure may cause unexpected negative consequences.
先前已有研究报道，脉冲磁场（PMF）治疗或抗 Ly6G 抗体的给药具有抗炎特性。在这项研究中，我们假设 PMF 治疗与抗 Ly6G 给药的联合可能会协同增强其抗炎作用。通过检查炎症部位的炎症迹象、组织病理学特性以及测量角叉菜胶诱导的急性爪炎症大鼠爪组织中巨噬细胞炎性蛋白-1α（MIP-1α/CCL3）和髓过氧化物酶（MPO）水平，研究了 PMF 治疗与抗 Ly6G 给药联合的效果。在本研究中，单独的 PMF 治疗或抗 Ly6G 的给药可改善急性炎症。然而，它们的联合使用加剧了炎症迹象、痛觉过敏、感觉异常、水肿和发热，并通过炎症细胞过度浸润到炎症部位加重了炎症状况。这些联合治疗的相反作用可能与炎症爪中 MIP-1α 和 MPO 水平的升高有关。目前的结果表明，PMF 治疗与抗 Ly6G 给药的联合可能不会带来额外的益处，实际上可能会导致急性炎症过程的恶化。这些发现还表明，在针对炎症状态的中性粒细胞或免疫细胞靶向治疗期间，磁场暴露可能会导致意想不到的负面后果。
