在十二烷基苯磺酸钠存在下，用聚（L-赖氨酸）修饰电极对甲氨蝶呤进行高灵敏度测定。
Highly sensitive determination of methotrexate at poly (l-lysine) modified electrode in the presence of sodium dodecyl benzene sulfonate.
机构信息
Department of Chemistry, Shanghai University, Shanghai 200444, PR China.
Department of Chemistry, Shanghai University, Shanghai 200444, PR China; School of Materials Science and Engineering, Shanghai University, Shanghai 200444, PR China.
出版信息
Bioelectrochemistry. 2014 Aug;98:70-5. doi: 10.1016/j.bioelechem.2014.03.005. Epub 2014 Mar 19.
A simple and sensitive electrochemical sensor based on poly (l-lysine) modified glassy carbon electrode (PLL/GCE) was developed to sensitively detect methotrexate (MTX) in the presence of sodium dodecyl benzene sulfonate (SDBS). Fourier transform infrared spectroscopy, electrochemical impedance spectroscopy and cyclic voltammetry were carried out to characterize PLL film which exhibits excellent electrocatalytic activity toward the oxidation of MTX in 0.1M phosphate buffer solution. In addition, the oxidation peak of MTX remained stable at PLL/GCE in the presence of SDBS and its current increased 8 times compared with that at bare GCE without SDBS. Experimental parameters were optimized with regard to pH, electro-polymerization segment, accumulation time and concentration of SDBS. Under optimum conditions, the square wave voltammograms exhibited that the oxidation peak current was linearly proportional to the concentration of MTX in the range of 5nM - 0.2μM with detection limit of 1.7 (±0.06) nM (S/N=3). Moreover, this method was applied to detect MTX in medicinal tablets with satisfying results.
基于聚赖氨酸（PLL）修饰玻碳电极（GCE）构建了一种简单灵敏的电化学传感器，用于在十二烷基苯磺酸钠（SDBS）存在下灵敏检测甲氨蝶呤（MTX）。采用傅里叶变换红外光谱、电化学阻抗谱和循环伏安法对 PLL 膜进行了表征，结果表明，PLL 膜在 0.1M 磷酸盐缓冲溶液中对 MTX 的氧化具有优异的电催化活性。此外，在 SDBS 存在下，MTX 在 PLL/GCE 上的氧化峰保持稳定，与无 SDBS 的裸 GCE 相比，其电流增加了 8 倍。实验参数通过 pH 值、电聚合段、富集时间和 SDBS 浓度进行了优化。在最佳条件下，方波伏安法显示，氧化峰电流在 5nM-0.2μM 范围内与 MTX 的浓度呈线性关系，检测限为 1.7（±0.06）nM（S/N=3）。此外，该方法还用于检测药用片中的 MTX，结果令人满意。
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