Teichmann J V, Sieber G, Ludwig W D, Ruehl H
Department of Hematology and Oncology, Klinikum Steglitz, Free University of Berlin, FRG.
J Interferon Res. 1988 Feb;8(1):15-24. doi: 10.1089/jir.1988.8.15.
Serial in vitro immune function studies of peripheral blood mononuclear cells (PBMC) were carried out during the long-term treatment with recombinant interferon-alpha 2 (IFN-alpha 2) in a patient with hairy-cell leukemia (HCL). Parameters of B- and T-cell functions as well as NK-cell activity were determined. Treatment with IFN-alpha 2 is associated with temporary and long-term depression of some immune functions, but can also normalize immune responses: in vitro-induced immunoglobulin synthesis, which was normal at diagnosis, was inhibited during the first weeks of IFN therapy but subsequently rose to normal levels. Lymphocyte proliferative responses to mitogens and antigens that were markedly reduced pretherapeutically were further depressed by IFN treatment but, with the exception of pokeweed mitogen (PWM)-induced responses, normalized completely by the 15th to 17th week of treatment. Cocultivation of PBMC with monocytes from normal individuals enhanced depressed lymphocyte proliferative responses. NK-cell activity, which was low at diagnosis, did not rise to the normal range during IFN treatment, but rapidly normalized when IFN therapy was stopped. A discussion is presented on the implications of the alteration of immune functions by treatment with IFN.
对一名毛细胞白血病（HCL）患者使用重组干扰素α2（IFN-α2）进行长期治疗期间，对外周血单个核细胞（PBMC）进行了系列体外免疫功能研究。测定了B细胞和T细胞功能参数以及NK细胞活性。IFN-α2治疗与某些免疫功能的暂时和长期抑制有关，但也可使免疫反应正常化：诊断时正常的体外诱导免疫球蛋白合成在IFN治疗的最初几周受到抑制，但随后升至正常水平。治疗前明显降低的对有丝分裂原和抗原的淋巴细胞增殖反应在IFN治疗后进一步受到抑制，但除了美洲商陆有丝分裂原（PWM）诱导的反应外，在治疗第15至17周时完全恢复正常。PBMC与正常个体的单核细胞共培养可增强受抑制的淋巴细胞增殖反应。诊断时较低的NK细胞活性在IFN治疗期间未升至正常范围，但在停止IFN治疗后迅速恢复正常。文中讨论了IFN治疗对免疫功能改变的影响。
