Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China.
Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
Food Chem. 2020 Nov 30;331:127090. doi: 10.1016/j.foodchem.2020.127090. Epub 2020 Jun 18.
Chlorothalonil is a class of 2B carcinogen which is widely used in the prevention and treatment of fungal diseases in food samples. Its residual problem has been increasingly concerned by society. In this paper, a fast and simple colorimetric assay based on Manganese dioxide nanosheets (MnO NSs)-oxidize 3,3',5,5'-tetramethylbenzidine (TMB) platform was used to detect residual pesticide chlorothalonil in food samples. Under optimal conditions, the half maximal inhibitory concentration and the limit of detection of chlorothalonil were 3.27 and 0.024 ng/mL. There were no obvious cross-reactivity between chlorothalonil and interference substances. The recoveries shown the satisfactory results. The results of colorimetric assay for the authentic samples were largely consistent with gas chromatography. Therefore, the proposed method would be convenient and satisfactory analytical methods for the monitoring of chlorothalonil. Furthermore, the MnO - TMB system was used to produce test strips for quick and convenient visual detection of chlorothalonil with good performance.
百菌清是一种广泛应用于食品样品真菌病防治的 2B 类致癌物质。其残留问题已引起社会的日益关注。在本文中，我们使用基于二氧化锰纳米片（MnO NSs）氧化 3,3',5,5'-四甲基联苯胺（TMB）平台的快速简便比色法来检测食品样品中的残留农药百菌清。在最佳条件下，百菌清的半抑制浓度和检测限分别为 3.27 和 0.024 ng/mL。百菌清与干扰物质之间没有明显的交叉反应。显色法测定的回收率结果令人满意。比色法对真实样品的测定结果与气相色谱法基本一致。因此，该方法将为百菌清的监测提供一种方便、令人满意的分析方法。此外，MnO-TMB 体系被用于制备测试条，用于快速方便地进行百菌清的目视检测，具有良好的性能。
