College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Mikrochim Acta. 2024 Oct 8;191(11):654. doi: 10.1007/s00604-024-06736-z.
By self-assembly of MnCl and arginine under alkaline conditions, ultra-small MnArg nanoparticles were successfully constructed as oxidase (OXD) mimics for intelligent detection of the Ginkgo toxin 4'-O-methylpyridoxal (MPN). The obtained MnArg nanozymes possessed excellent OXD-like activity and thermal stability. Based on the inhibitory effect of MPN for the catalytic activity of MnArg, this system was utilized for the colorimetric sensing of MPN with a low limit of detection (LOD) of 2.16 µg mL. The detection system exhibited good selectivity against other potential interferents. FTIR data showed that the presence of MPN binds with MnArg and shields the active sites, thereby interfering with the oxidase-like activity. Combined with a smartphone and the ColorMax software, this nanozyme-based intelligent detection platform could effectively detect MPN with a LOD of 2.1 µg mL. Our MnArg nanozyme-based system was applied to detect real ginkgo nut samples with recoveries of 92.4-108.7%, and the relative standard deviations were less than 0.7%. This work may promote the development of novel nanozymes and expand their applications in the field of food safety detection.
通过在碱性条件下自组装 MnCl 和精氨酸，成功构建了超小的 MnArg 纳米颗粒作为氧化酶 (OXD) 模拟物，用于银杏毒素 4'-O-甲基吡哆醛 (MPN) 的智能检测。所得的 MnArg 纳米酶具有优异的 OXD 样活性和热稳定性。基于 MPN 对 MnArg 催化活性的抑制作用，该系统用于 MPN 的比色检测，检测限 (LOD) 低至 2.16 µg mL。该检测系统对其他潜在干扰物具有良好的选择性。FTIR 数据表明，MPN 的存在与 MnArg 结合并屏蔽了活性位点，从而干扰了氧化酶样活性。结合智能手机和 ColorMax 软件，该基于纳米酶的智能检测平台可以有效地检测 MPN，检测限为 2.1 µg mL。我们的基于 MnArg 纳米酶的系统用于检测真实的银杏坚果样品，回收率为 92.4-108.7%，相对标准偏差小于 0.7%。这项工作可能会促进新型纳米酶的发展，并拓展它们在食品安全检测领域的应用。
