Department of Fisheries and Diseases, Faculty of Veterinary Medicine, Aksaray University, Turkey.
Department of Veterinary Pathology, Faculty of Veterinary Medicine, Aksaray University, Turkey.
J Trace Elem Med Biol. 2020 Dec;62:126581. doi: 10.1016/j.jtemb.2020.126581. Epub 2020 Jun 18.
Even though boron (B), as a trace micronutrient, occurs in natural waters and organisms, its high concentration could cause harmful and even toxic for organisms. The aim of present study was to investigate the effects of boric acid (BA) added to feed (0.01%, 0.05%, 0.10%, and 0.20% of B in feed) on Rainbow Trout (Oncorhynchus mykiss) by histopathological methods and compared to the control feed as without B.
At the end of the feeding, after weighing the live weight of 9 rainbow trout from each group, tissue fragments were taken from the liver, gill, kidney, skeletal muscle, spleen and brains of fish, which systemic necropsies was done, and were fixed in 10 % buffered formalin solution.
Histopathological examinations revealed degenerative and necrotic changes in the liver, gill, kidney, skeletal muscle, spleen and brain. Hydropic and vacuolar degenerations in liver parenchyma, lamellar edema in the gills, hyaline accumulation in the kidneys, degenerations in the muscles, necrosis in the spleen and hyperemia in the brain were observed in all groups except control group. It was observed that the destruction of boric acid on fish increased depending on the amount of boron supplemented to the feed, and the most effect was in the group fed with 0.20 % boron supplemented feed.
The findings aside from causing pathological changes in all organs in terms of histopathological findings of Boric Acid (BA), the most severe lesions were observed in the liver.
尽管硼（B）作为一种痕量微量元素存在于天然水和生物体中，但高浓度的硼可能对生物体造成有害甚至有毒的影响。本研究旨在通过组织病理学方法研究添加到饲料中的硼酸（BA）（饲料中 B 的 0.01%、0.05%、0.10%和 0.20%）对虹鳟（Oncorhynchus mykiss）的影响，并与不含 B 的对照饲料进行比较。
在喂食结束后，从每组 9 条虹鳟中称重活体重量后，从鱼的肝脏、鳃、肾脏、骨骼肌、脾脏和大脑中取出组织碎片，进行系统尸检，并固定在 10%缓冲福尔马林溶液中。
组织病理学检查显示肝脏、鳃、肾脏、骨骼肌、脾脏和大脑发生退行性和坏死性变化。除对照组外，所有组均观察到肝实质出现水肿和空泡变性、鳃板水肿、肾脏透明质积聚、肌肉变性、脾脏坏死和大脑充血。观察到随着饲料中补充硼量的增加，硼酸对鱼的破坏增加，而在补充 0.20%硼的饲料组中观察到的影响最大。
除了硼酸（BA）在所有器官的组织病理学发现中引起病理变化外，肝脏观察到的病变最严重。
