Department of Restorative Dentistry, Rutgers School of Dental Medicine, Newark, New Jersey; Department of Endodontics, University of the Pacific, Arthur A. Dugoni School of Dentistry, San Francisco, California.
Sector of Angiogenesis Regenerative Medicine, Dr. Hajar Afsar Lajevardi Dental Material and Devices Group, Hackensack, New Jersey.
J Endod. 2020 Aug;46(8):1113-1119. doi: 10.1016/j.joen.2020.04.005. Epub 2020 Jun 24.
This study evaluated the effect of different pH values of 4.4, 5.4, 6.4, 7.4, 8.4, and 9.4 on angiogenesis.
Endothelial cells were isolated from the mice molar teeth and placed in 42 Matrigel (Corning, NY)-coated wells, which were prepared and divided into 6 groups (n = 7). Synthetic tissue fluid was prepared and divided into 6 parts, and their pH values were adjusted to 4.4, 5.4, 6.4, 7.4, 8.4, and 9.4. A 2-mL volume from each group was diluted in the growth medium at a ratio of 1:3 and used for tubulogenesis assay. Forty-two 6-week-old mice in 6 groups (n = 7) were used for choroidal neovascularization (CNV). A 2-μL volume from each group or saline (control) was delivered by intravitreal injection on the day of laser application and 1 week later. Data on the number of nodes, the total length of the branches, and CNV areas (μm) were determined using ImageJ software (National Institutes of Health, Bethesda, MD) and analyzed with 1-way analysis of variance and post hoc Tukey tests. The correlation was assessed between the tested variables.
The number of nodes decreased with changes in pH values as follows: 6.4 > 5.4 > 7.4 > 8.4 > 9.4 > 4.4. The total branch length decreased with pH value changes as follows: 6.4 > 4.4 > 6.4 > 7.4 > 8.4 > 9.4, and the CNV areas decreased with pH value changes as follows: 6.4 > 5.4 > 4.4 > 7.4 > 8.4 > 9.4.
Moderately acidic pH values (5.4 and 6.4) enhanced angiogenesis, whereas moderately alkaline pH values (8.4 and 9.4) suppressed angiogenesis.
本研究评估了不同 pH 值（4.4、5.4、6.4、7.4、8.4 和 9.4）对血管生成的影响。
从老鼠磨牙中分离出内皮细胞，置于 42 孔 Matrigel（康宁，纽约）包被的孔中，将其制备并分为 6 组（n = 7）。制备合成组织液并分为 6 份，将其 pH 值分别调整为 4.4、5.4、6.4、7.4、8.4 和 9.4。将每组的 2 毫升体积用生长培养基按 1:3 的比例稀释，用于小管生成测定。将 6 组（n = 7）的 42 只 6 周龄小鼠用于脉络膜新生血管（CNV）。在激光应用当天和 1 周后，每组通过眼内注射 2μL 体积的各组或生理盐水（对照）。使用 ImageJ 软件（美国国立卫生研究院，贝塞斯达，MD）确定节点数、分支总长度和 CNV 面积（μm）的数据，并使用单因素方差分析和事后 Tukey 检验进行分析。评估了测试变量之间的相关性。
节点数随 pH 值的变化而减少，如下所示：6.4>5.4>7.4>8.4>9.4>4.4。总分支长度随 pH 值变化而减少，如下所示：6.4>4.4>6.4>7.4>8.4>9.4，CNV 面积随 pH 值变化而减少，如下所示：6.4>5.4>4.4>7.4>8.4>9.4。
适度酸性 pH 值（5.4 和 6.4）增强血管生成，而适度碱性 pH 值（8.4 和 9.4）抑制血管生成。
