Trushinskaia G N, Gubareva L V, Parasiuk N A, Ivanova V T, Zhadanov V M
Vopr Virusol. 1988 Jan-Feb;33(1):11-7.
Changes in the activity of natural cytotoxic lymphocytes (natural killers, NK) were studied in the course of infection of CBA mice with a mouse-adapted or unadapted (epidemic) variants of influenza A/USSR/90/77 virus. The activity of NK against K562 target cells in the peripheral blood, lungs, and spleens was found to increase considerably 18 hours after intranasal inoculation with any of the variants and to persist at a high level for up to 72 hours. Virus replication in the lungs and peripheral blood was observed only in mice infected with the adapted virus variant. A conclusion was drawn that the increase in NK activity in the organs of influenza virus-infected mice was nonspecific providing no protection against a fatal outcome after infection with the mouse-adapted (pathogenic) virus. Treatment of lymphocytes recovered from the peripheral blood, lungs, and spleens of infected and non-infected mice in vitro with preparations of intact viruses and isolated viral proteins exerted different effects on the NK activity: enhancement after treatment with intact viruses and inhibition by isolated viral proteins. The effect did not depend on the virulence of the strain from which viral proteins were isolated. The importance of the phenomenon of different modulating effect of influenza virus and its structural proteins on the NK activity is discussed.
研究了用甲型流感病毒A/USSR/90/77的小鼠适应株或未适应株（流行株）感染CBA小鼠过程中自然细胞毒性淋巴细胞（自然杀伤细胞，NK）活性的变化。发现用任何一种病毒株经鼻内接种18小时后，外周血、肺和脾脏中NK对K562靶细胞的活性显著增加，并在高水平持续长达72小时。仅在感染适应病毒株的小鼠中观察到病毒在肺和外周血中的复制。得出的结论是，流感病毒感染小鼠器官中NK活性的增加是非特异性的，不能为感染小鼠适应株（致病株）后的致命结局提供保护。用完整病毒制剂和分离的病毒蛋白体外处理从感染和未感染小鼠的外周血、肺和脾脏中回收的淋巴细胞，对NK活性产生不同影响：用完整病毒处理后增强，而分离的病毒蛋白则抑制。该效应不取决于分离病毒蛋白的毒株的毒力。讨论了流感病毒及其结构蛋白对NK活性的不同调节作用现象的重要性。
