Mak N K, Sweet C, Ada G L, Tannock G A
Immunology. 1984 Feb;51(2):407-16.
Reductions in virus titres and the generation of enhanced cytotoxic T cell (Tc) activity in the lungs of mice primed either with a wild-type, parental (H2N2) influenza virus, A/AA/6/60, or a cold-adapted variant A/AA/6/60-ca and challenged 6 weeks later with a H1N1 A/WSN virus showed that both H2N2 viruses could sensitize the mice. A comparison of graded sensitizing doses of each virus showed that inocula of 10(6) tissue culture infective doses (TCID50) of the ca-variant or 10(3) TCID50 of the wild-type virus gave similar results. The spleens and lungs of normal mice were found to contain similar levels (circa 1/10(5) cells) of precursor Tc cells and the level in the lung did not increase 2 days after intranasal (i.n.) inoculation of A/WSN virus. Two and 6 weeks after priming mice with 10(5) TCID50 of either virus, the lungs contained about a 20-fold increase in the precursor Tc cell frequency. In contrast, sensitization with a sub-lethal dose of a mouse-adapted A/WSN virus caused a 100-fold or greater increase. Sensitization of mice with the parental but not the ca-variant virus caused an increase in frequency of precursor Tc cells in the spleens of the sensitized mice and this might reflect the very low level of replication of the ca-variant virus in the mouse lung.
用野生型亲代（H2N2）流感病毒A/AA/6/60或冷适应变体A/AA/6/60 - ca对小鼠进行初次免疫，6周后用H1N1 A/WSN病毒攻击，结果显示两种H2N2病毒均可使小鼠致敏，小鼠肺部病毒滴度降低，细胞毒性T细胞（Tc）活性增强。对每种病毒不同致敏剂量的比较表明，10(6)组织培养感染剂量（TCID50）的冷适应变体或10(3) TCID50的野生型病毒接种产生了相似的结果。发现正常小鼠的脾脏和肺中含有相似水平（约1/10(5)个细胞）的前体Tc细胞，鼻内接种A/WSN病毒2天后肺中的水平并未增加。用10(5) TCID50的任何一种病毒对小鼠进行初次免疫2周和6周后，肺中前体Tc细胞频率增加了约20倍。相比之下，用亚致死剂量的小鼠适应型A/WSN病毒致敏导致增加100倍或更多。用亲代病毒而非冷适应变体病毒致敏小鼠会导致致敏小鼠脾脏中前体Tc细胞频率增加，这可能反映了冷适应变体病毒在小鼠肺中的复制水平非常低。
