Department of Clinical Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.; Institute of Biomedical Research, University of Tehran, Tehran, Iran; Postal Code: 1419963111.
Res Vet Sci. 2020 Oct;132:186-193. doi: 10.1016/j.rvsc.2020.04.018. Epub 2020 May 17.
1, 25-dihydroxycholecalciferol is recognized as a potent immune-modulator which can fight against the pathogens via the activation of vitamin D3 receptors (VDRs), as well as stimulating various cytokines in infectious diseases. In the present study, because of the vitamin D3 has an appropriate immunomodulatory, the effects of this vitamin on the levels of pre-inflammatory and anti-inflammatory cytokines have been investigated in calves with experimental pasteurellosis. This study was experimentally carried out on 10 Holstein crossbred male calves (2-4 months) that were divided into two groups. Prepared Pasteurella multocida (3 × 10 CFU/mL) was inoculated in the trachea with a lavage catheter and then the treatment group was injected with 1, 25-dihydroxycholecalciferol after confirming pneumonia. Blood sampling, clinical symptoms scoring and radiological evaluation were recorded for both groups at different time intervals. The prescription of, vitamin D to the treatment group caused a decline in clinical symptoms score and changed interstitial and alveolo-interstitial lung pattern to such a degree that it could recover in comparison with the control group. The concentrations of pro-inflammatory cytokines (i.e., IL-1β, IL-6, and TNF-α) and the chemokine (IL-8) showed a significant decrease in the treatment group while the concentration of IL-10 increased in the treatment groups following the vitamin D injection (P = .001). The evidence from the current study suggests that vitamin D3 exert the immunomodulatory effects in infectious diseases through the regulation of cytokines and activation of VDR pathways to produce antimicrobial peptides.
1,25-二羟胆钙化醇被认为是一种有效的免疫调节剂，它可以通过激活维生素 D3 受体 (VDR) 以及刺激感染性疾病中的各种细胞因子来对抗病原体。在本研究中，由于维生素 D3 具有适当的免疫调节作用，因此研究了这种维生素对实验性巴氏杆菌病牛犊前炎症和抗炎细胞因子水平的影响。这项研究是在 10 头荷斯坦杂交雄性小牛（2-4 个月）中进行的，这些小牛被分为两组。用灌洗导管将巴氏杆菌（3×10 CFU/mL）接种到气管中，然后在确认肺炎后，治疗组注射 1,25-二羟胆钙化醇。在不同的时间间隔，对两组进行了采血、临床症状评分和放射学评估。向治疗组开处方维生素 D 可降低临床症状评分，并使间质性和肺泡间质性肺模式发生变化，与对照组相比，这种变化可以恢复。在治疗组中，促炎细胞因子（即 IL-1β、IL-6 和 TNF-α）和趋化因子（IL-8）的浓度显著降低，而治疗组中 IL-10 的浓度在注射维生素 D 后增加（P=0.001）。本研究的证据表明，维生素 D3 通过调节细胞因子和激活 VDR 途径产生抗菌肽，在传染病中发挥免疫调节作用。
