Department of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, ul. Borowska 211 A, 50-556, Wrocław, Poland.
Department of Microbiology, Wroclaw Medical University, ul. T. Chałubińskiego 4, 50-368, Wrocław, Poland.
Biomed Pharmacother. 2020 Sep;129:110435. doi: 10.1016/j.biopha.2020.110435. Epub 2020 Jun 25.
In this study, we evaluated antimicrobial activity, antimicrobial activity in combination with antibiotics, and chemical composition of Nepalese propolis 70% ethanolic extracts. Propolis originated from two genera of bees - Apis mellifera L. and Trigona sp. HPLC-DAD-MS/MS analyses revealed that the composition of both extracts was almost the same and the main components were flavonoid aglycones (mainly neoflavonoids, isoflavonoids) and pterocarpans. The highest antibacterial activity (disc diffusion test) was observed against Helicobacter pylori, Staphylococcus aureus and Shigella flexneri. Antibiotics exhibited synergism with Apis mellifera L. and Trigona sp. propolis against S. aureus and the strongest effect was observed for the combination with amikacin and tetracycline. Moreover, Nepalase propolis inhibited filamentation of C. albicans and caused oxidative stress by production of the superoxide anion radical (O) and a lower concentration of the hydroxyl radical (OH). Propolis extracts are potent antibacterial agents and may be used in combination with antibiotics.
在这项研究中，我们评估了尼泊尔蜂胶 70%乙醇提取物的抗菌活性、与抗生素联合的抗菌活性以及化学成分。蜂胶来源于蜜蜂属的两种蜜蜂 - 意大利蜜蜂和 Trigona 属。HPLC-DAD-MS/MS 分析表明，两种提取物的成分几乎相同，主要成分是类黄酮苷元（主要是新黄酮、异黄酮）和紫檀烷。对幽门螺杆菌、金黄色葡萄球菌和福氏志贺菌的抗菌活性（圆盘扩散试验）最高。抗生素与意大利蜜蜂和 Trigona 属蜂胶表现出协同作用，对金黄色葡萄球菌的作用最强，与阿米卡星和四环素的组合作用最强。此外，尼泊尔蜂胶抑制白色念珠菌的丝状生长，并通过产生超氧阴离子自由基 (O) 和较低浓度的羟基自由基 (OH) 引起氧化应激。蜂胶提取物是有效的抗菌剂，可与抗生素联合使用。
