Miorin P L, Levy Junior N C, Custodio A R, Bretz W A, Marcucci M C
Instituto de Ciências Biomédicas da Universidade de São Paulo, São Paulo, SP, Brazil.
J Appl Microbiol. 2003;95(5):913-20. doi: 10.1046/j.1365-2672.2003.02050.x.
The antibacterial activity against Staphylococcus aureus of honey and propolis produced by Apis mellifera and Tetragonisca angustula was evaluated. Secondary aims included the study of the chemical composition of propolis and honey samples and its relationship with antibacterial activity against S. aureus.
The antibacterial activity of honey and propolis was determined by the method of macrodilution. The minimum inhibitory concentration (MICs) of A. mellifera honey ranged from 126.23 to 185.70 mg ml(-1) and of T. angustula from 142.87 to 214.33 mg ml(-1). For propolis, the MIC ranged from 0.36 to 3.65 mg ml(-1) (A. mellifera) and from 0.44 to 2.01 mg ml(-1) (T. angustula). Honey and propolis were evaluated by high-performance liquid chromatography. Some typical compounds of Brazilian propolis were also identified in honey samples. Principal component analysis revealed that the chemical composition of honey and propolis samples was distinct based on the geographical location of the samples.
Propolis samples had higher antibacterial activity against S. aureus when compared with honey. However, both propolis and honey samples had antibacterial against S. aureus.
These antimicrobial properties would warrant further studies on the clinical applications of propolis and honey against S. aureus.
评估意大利蜜蜂（Apis mellifera）和小蜜蜂（Tetragonisca angustula）所产蜂蜜和蜂胶对金黄色葡萄球菌的抗菌活性。次要目的包括研究蜂胶和蜂蜜样品的化学成分及其与对金黄色葡萄球菌抗菌活性的关系。
采用宏观稀释法测定蜂蜜和蜂胶的抗菌活性。意大利蜜蜂蜂蜜的最低抑菌浓度（MICs）范围为126.23至185.70 mg/ml，小蜜蜂蜂蜜的最低抑菌浓度范围为142.87至214.33 mg/ml。对于蜂胶，意大利蜜蜂蜂胶的MIC范围为0.36至3.65 mg/ml，小蜜蜂蜂胶的MIC范围为0.44至2.01 mg/ml。通过高效液相色谱法对蜂蜜和蜂胶进行评估。在蜂蜜样品中还鉴定出了一些巴西蜂胶的典型化合物。主成分分析表明，根据样品的地理位置，蜂蜜和蜂胶样品的化学成分不同。
与蜂蜜相比，蜂胶样品对金黄色葡萄球菌具有更高的抗菌活性。然而，蜂胶和蜂蜜样品均对金黄色葡萄球菌有抗菌作用。
这些抗菌特性将促使对蜂胶和蜂蜜针对金黄色葡萄球菌的临床应用进行进一步研究。
