Quintero-Mora María Leonor, Londoño-Orozco Amparo, Hernández-Hernández Francisca, Manzano-Gayosso Patricia, López-Martínez Rubén, Soto-Zárate Carlos Ignacio, Carrillo-Miranda Liborio, Penieres-Carrillo Guillermo, García-Tovar Carlos Gerardo, Cruz-Sánchez Tonatiuh A
Facultad de Estudios Superiores Cuautitlán, Departamento de Microbiología y Parasitología, Universidad Nacional Autónoma de México, Mexico.
Rev Iberoam Micol. 2008 Mar;25(1):22-6. doi: 10.1016/s1130-1406(08)70006-8.
Propolis is a resinous substance collected by bees (Apis mellifera) from different trees and bushes. Due to its antifungal, antibacterial, antiviral and antiparasitic properties, it has continued to be very popular throughout the time showing variable activity depending on its geographical origin. In Mexico, information about this product is very limited. The aim of this work was to evaluate the antifungal activity of four propolis ethanolic extracts from three different Mexican states, and four commercial extracts on Candida albicans growth. A reference strain (ATCC 10231) and 36 clinical isolates of C. albicans were used. The Minimal Inhibitory Concentration (MIC) was determined by the dilution on agar method. Growth curves on Sabouraud Dextrose broth with and without different propolis ethanolic extracts concentrations were performed. In addition, whether the effect was fungistatic or fungicide was determined. The propolis ethanolic extract obtained from Cuautitlán Izcalli, State of Mexico, showed the best biological activity, inhibiting 94.4% from the clinical isolates at 0.8 mg/ml; the reference strain was inhibited at 0.6 mg/ml. The propolis effect was fungistatic in low concentrations and fungicide in concentrations higher to MIC. The Mexican propolis ethanolic extract could be further investigated for its alternative use for the treatment of some C. albicans infections.
蜂胶是蜜蜂（意大利蜜蜂）从不同树木和灌木丛中采集的树脂状物质。由于其具有抗真菌、抗菌、抗病毒和抗寄生虫特性，一直以来都很受欢迎，但其活性因地理来源而异。在墨西哥，关于这种产品的信息非常有限。这项工作的目的是评估来自墨西哥三个不同州的四种蜂胶乙醇提取物以及四种商业提取物对白色念珠菌生长的抗真菌活性。使用了一株参考菌株（ATCC 10231）和36株白色念珠菌临床分离株。通过琼脂稀释法测定最低抑菌浓度（MIC）。在含有和不含有不同浓度蜂胶乙醇提取物的沙氏葡萄糖肉汤中进行生长曲线实验。此外，还确定了其作用是抑菌还是杀菌。从墨西哥州库阿蒂特兰伊萨利获得的蜂胶乙醇提取物显示出最佳的生物活性，在0.8毫克/毫升时抑制了94.4%的临床分离株；参考菌株在0.6毫克/毫升时被抑制。蜂胶在低浓度时具有抑菌作用，在高于MIC的浓度时具有杀菌作用。墨西哥蜂胶乙醇提取物可进一步研究其在治疗某些白色念珠菌感染方面的替代用途。
