Vo Thuy-Lan Thi, Yang Nae-Cherng, Yang Shu-Er, Chen Chien-Lin, Wu Chi-Hao, Song Tuzz-Ying
Department of Food Science and Biotechnology, College of Biotechnology and Bioresources, Da-Yeh University, Changhua, Taiwan.
Department of Nutrition, Chung Shan Medical University, Taichung, Taiwan.
Chin J Physiol. 2020 May-Jun;63(3):137-148. doi: 10.4103/CJP.CJP_88_19.
Cajanus cajan (L.) Millsp., also named pigeon pea, is widely grown in the tropics and the subtropics. C. cajan roots (CR) and ribs stewed in hot water have been used as a traditional medicine in various cultures to treat diabetes. The purpose of this study was to determine the functional components of hot water (WCR) and 50%, 95% ethanol extracts (EECR50 and EECR95) from CR, then evaluating their antioxidant and anti-inflammatory effects. The results indicated that EECR95 had higher polyphenol, especially the isoflavones (e.x. daidzein, genistein, and cajanol) than those of the other extracts, and it also exhibited the most potent anti-oxidative activities by in vitro antioxidant assay. In the lipopolysaccharide-stimulated RAW 264.7 cells, we found that EECR95 significantly decreased intracellular reactive oxygen species and significantly enhanced the activities of superoxide dismutase and catalase. Mechanism studies showed that EECR95 mainly activated nuclear factor (NF) erythroid 2-related factor 2/antioxidant protein heme oxygenase-1 and inhibited nuclear factor kappa B (NF-κB) signaling pathway, and thus exhibited antioxidant and anti-inflammatory effects. Overall, this study suggests that CR may have the potential to be developed as a biomedical material and that genistein, which has relatively high uptakes (3.44% for the pure compound and 1.73% for endogenous genistein of EECR95) at 24 h of incubation with RAW 264.7 cells, could be the main active component of CR.
木豆（Cajanus cajan (L.) Millsp.），也被称为鸽豆，广泛种植于热带和亚热带地区。木豆根（CR）及热水炖排骨在不同文化中一直被用作治疗糖尿病的传统药物。本研究旨在确定木豆根热水提取物（WCR）以及50%、95%乙醇提取物（EECR50和EECR95）的功能成分，然后评估它们的抗氧化和抗炎作用。结果表明，EECR95比其他提取物含有更高的多酚，尤其是异黄酮（如大豆苷元、染料木黄酮和木豆醇），并且通过体外抗氧化试验显示其具有最强的抗氧化活性。在脂多糖刺激的RAW 264.7细胞中，我们发现EECR95显著降低细胞内活性氧水平，并显著增强超氧化物歧化酶和过氧化氢酶的活性。机制研究表明，EECR95主要激活核因子（NF）红细胞2相关因子2/抗氧化蛋白血红素加氧酶-1，并抑制核因子κB（NF-κB）信号通路，从而发挥抗氧化和抗炎作用。总体而言，本研究表明木豆根可能有潜力被开发为生物医学材料，并且在与RAW 264.7细胞孵育24小时时摄取量相对较高（纯化合物为3.44%，EECR95的内源性染料木黄酮为1.73%）的染料木黄酮可能是木豆根的主要活性成分。
