Department of Geriatric Medicine, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India.
Department of Biostatistics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India.
Aging Clin Exp Res. 2021 May;33(5):1315-1324. doi: 10.1007/s40520-020-01642-9. Epub 2020 Jun 27.
The aging trajectory from a state of robustness and good health proceeds from sarcopenia to frailty followed by disability and death due to decline in skeletal muscle mass and function. Sarcopenia is now formally recognized as a muscle disease with an ICD-10-MC diagnosis code. The autophagic response seems to be affected in the skeletal muscle during aging contributing to sarcopenia. Sestrins (Sesns) proteins play a critical role in autophagy induction under cellular stress conditions.
The study aims to identify sarcopenia in older adults using Asian Working group guidelines (AWGS) to determine clinically relevant cut-off levels for diagnosis and their association with antioxidant protein Sesns.
The study recruited 102 older adults attending Geriatric medicine OPD AIIMS, New Delhi, India. The level of serum Sesns were evaluated by Surface Plasmon Resonance (SPR) and validated by immunoblotting. Fifty older adults were diagnosed as sarcopenics according to AWGS.
Sesn 1 (p = 0.0448) and Sesn 2 (p < 0.0001) levels were significantly reduced in sarcopenic compared to non-sarcopenic. ROC analysis showed a better cut-off of Sesn 2; 10.104 ng/µL with 92% sensitivity and 84% specificity. Even after adjusting the values with respect to confounding factors, Sesn levels remained significantly reduced in sarcopenics (p < 0.030).
The level of Sesn 2 showed positive co-relation with the characteristics of sarcopenia. This study first time reported the concentration of serum sestrin in sarcopenic older adults.
It can be concluded that sarcopenia can be diagnosed at the early stage by using the serum sestrin scale as one of the potential biomarker.
从健壮和健康状态到衰弱，再到因骨骼肌质量和功能下降而导致的残疾和死亡，衰老轨迹是一个渐进的过程。肌少症现在被正式认定为一种肌肉疾病，有 ICD-10-MC 诊断代码。衰老过程中，骨骼肌的自噬反应似乎受到影响，导致肌少症。Sesns（Sesn 蛋白）在细胞应激条件下的自噬诱导中发挥着关键作用。
本研究旨在使用亚洲工作组指南（AWGS）确定老年人中的肌少症，以确定用于诊断的临床相关临界值及其与抗氧化蛋白 Sesns 的关联。
该研究招募了 102 名在印度新德里 AIIMS 老年医学门诊就诊的老年人。通过表面等离子体共振（SPR）评估血清 Sesns 水平，并通过免疫印迹进行验证。根据 AWGS，50 名老年人被诊断为肌少症。
与非肌少症患者相比，Sesn1（p=0.0448）和 Sesn2（p<0.0001）水平在肌少症患者中显著降低。ROC 分析显示 Sesn2 的更好截断值为 10.104ng/µL，灵敏度为 92%，特异性为 84%。即使在考虑混杂因素后对值进行调整，肌少症患者的 Sesn 水平仍显著降低（p<0.030）。
Sesn2 的水平与肌少症的特征呈正相关。本研究首次报道了肌少症老年患者血清 sestrin 的浓度。
可以得出结论，通过使用血清 sestrin 量表作为潜在生物标志物之一，可以在早期诊断肌少症。
