Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), 489040 Leioa, Bizkaia, Spain.
Biocruces Bizkaia Health Research Institute, 48903 Barakaldo, Bizkaia, Spain.
Int J Environ Res Public Health. 2022 Jan 19;19(3):1079. doi: 10.3390/ijerph19031079.
Given the increasing prevalence of frailty and its implications for public health, the identification of biomarkers to detect frailty is essential. Sestrin-1 is a protein with a protective role in muscle function. This study aimed to determine whether the serum sestrin-1 concentration differed between frail and non-frail populations and to investigate its association with frailty-related variables in 225 older women and men living in nursing homes (Gipuzkoa, Spain). Serum sestrin-1 concentration was measured by ELISA. Frailty, dependence, anthropometry, physical function, and physical activity were determined by validated tests and tools. The associations between sestrin-1 concentration and the other variables were determined using generalized linear models. The differences between frail and non-frail individuals were analyzed by the Mann-Whitney U-test, and receiver operating characteristic (ROC) curves were constructed to calculate the capability of sestrin-1 to detect frailty. Unexpectedly, frail individuals-according to the Fried Frailty Phenotype or the Clinical Frailty Scale-had higher serum sestrin-1 concentrations than non-frail individuals. Furthermore, the higher serum sestrin-1 concentration was associated with the increased frailty scores and dependence as well as the poorer physical function and the less physical activity. Given the contradictory results regarding serum sestrin-1 and frailty, further investigation is required to propose it as a molecular biomarker of frailty.
鉴于衰弱的发病率不断上升及其对公共卫生的影响，确定可用于检测衰弱的生物标志物至关重要。Ses 蛋白-1 是一种具有肌肉功能保护作用的蛋白质。本研究旨在确定血清 Ses 蛋白-1 浓度在衰弱和非衰弱人群之间是否存在差异，并在居住在养老院的 225 名老年男女中（西班牙吉普斯夸），调查其与衰弱相关变量的关系。通过 ELISA 法测定血清 Ses 蛋白-1 浓度。通过经过验证的测试和工具确定衰弱、依赖、人体测量学、身体机能和身体活动。使用广义线性模型确定 Ses 蛋白-1 浓度与其他变量之间的关联。通过曼-惠特尼 U 检验分析衰弱和非衰弱个体之间的差异，并构建接收者操作特征（ROC）曲线以计算 Ses 蛋白-1 检测衰弱的能力。出乎意料的是，根据 Fried 衰弱表型或临床衰弱量表，衰弱个体的血清 Ses 蛋白-1 浓度高于非衰弱个体。此外，较高的血清 Ses 蛋白-1 浓度与增加的衰弱评分和依赖性以及较差的身体机能和较少的身体活动相关。鉴于血清 Ses 蛋白-1 与衰弱之间的矛盾结果，需要进一步研究以提出它作为衰弱的分子生物标志物。
