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采用 CHROMagar 定向培养基和快速生化试验评估肠杆菌科的推定鉴定。

Evaluation of presumptive identification of Enterobacterales using CHROMagar Orientation medium and rapid biochemical tests.

机构信息

Department of Clinical Laboratory Science, Graduate School of Kansai University of Health Sciences, Osaka, Japan.

Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu, Japan.

出版信息

J Clin Lab Anal. 2020 Oct;34(10):e23453. doi: 10.1002/jcla.23453. Epub 2020 Jun 28.

DOI:10.1002/jcla.23453
PMID:32594571
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7595914/
Abstract

BACKGROUND

The use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is gradually spreading among large-scale laboratories; however, this method is impractical for small-scale laboratories. In laboratories without access to these rapid identification methods, problems related to them remain unsolved. In this study, we aimed to develop a rapid and inexpensive method to presumptively identify Enterobacterales using CHROMagar Orientation medium.

METHODS

The algorithm for presumptive identification of Enterobacteriaceae using CHROMagar Orientation medium was based on our previous studies. Modified property tests for indole, lysine decarboxylase, ornithine decarboxylase, and hydrogen sulfide were performed to evaluate the differentiation of the bacterial species.

RESULTS

Using the type strains and clinical isolates, it was possible to conduct the property tests at a low cost, within 4 hours. The spot indole test was performed without any nonspecific reactions for the bacteria forming colored colonies. The presumptive identification of bacteria was thereby possible within 24 hours after specimen submission.

CONCLUSION

All these results suggest that the rapid presumptive identification of Enterobacterales is possible with this new identification method using CHROMagar Orientation medium. This is therefore a prompt and economical method that can be used in routine laboratory work.

摘要

背景

基质辅助激光解吸电离飞行时间质谱分析的使用在大型实验室中逐渐普及，但对于小型实验室来说，这种方法并不实用。在没有这些快速鉴定方法的实验室中，与之相关的问题仍然没有得到解决。本研究旨在开发一种使用 CHROMagar 定向培养基快速且廉价的方法，对肠杆菌目进行初步鉴定。

方法

使用 CHROMagar 定向培养基对肠杆菌科进行初步鉴定的算法基于我们之前的研究。对吲哚、赖氨酸脱羧酶、鸟氨酸脱羧酶和硫化氢进行了改良的特性试验，以评估细菌种类的分化。

结果

使用标准菌株和临床分离株，在 4 小时内即可进行成本低廉的特性试验。显色菌落的吲哚斑点试验没有出现任何非特异性反应。因此，在标本提交后 24 小时内即可进行细菌的初步鉴定。

结论

所有这些结果表明，使用 CHROMagar 定向培养基的这种新鉴定方法可以快速初步鉴定肠杆菌目。因此，这是一种快速且经济的方法，可用于常规实验室工作。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/479a/7595914/d1a3269b50a1/JCLA-34-e23453-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/479a/7595914/d1a3269b50a1/JCLA-34-e23453-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/479a/7595914/d1a3269b50a1/JCLA-34-e23453-g001.jpg

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