Nomura Fumio
Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Divisions of Laboratory Medicine, Clinical Genetics and Proteomics, Chiba University Hospital, 1-8-1 Inohana, Chiba City, Chiba 260-8670, Japan.
Biochim Biophys Acta. 2015 Jun;1854(6):528-37. doi: 10.1016/j.bbapap.2014.10.022. Epub 2014 Nov 1.
Rapid and accurate identification of microorganisms, a prerequisite for appropriate patient care and infection control, is a critical function of any clinical microbiology laboratory. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a quick and reliable method for identification of microorganisms, including bacteria, yeast, molds, and mycobacteria. Indeed, there has been a revolutionary shift in clinical diagnostic microbiology. In the present review, the state of the art and advantages of MALDI-TOF MS-based bacterial identification are described. The potential of this innovative technology for use in strain typing and detection of antibiotic resistance is also discussed. This article is part of a Special Issue entitled: Medical Proteomics.
快速准确地鉴定微生物是任何临床微生物实验室的一项关键功能，也是恰当的患者护理和感染控制的前提条件。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）是一种快速可靠的微生物鉴定方法，可用于鉴定细菌、酵母、霉菌和分枝杆菌等微生物。事实上，临床诊断微生物学已经发生了革命性的转变。在本综述中，描述了基于MALDI-TOF MS的细菌鉴定的技术现状和优势。还讨论了这项创新技术在菌株分型和抗生素耐药性检测中的应用潜力。本文是名为《医学蛋白质组学》的特刊的一部分。
