Department of Ophthalmology, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, Katowice, Poland.
Department of Ophthalmology, Kornel Gibiński University Clinical Center, Medical University of Silesia, Katowice, Poland.
Cutan Ocul Toxicol. 2020 Sep;39(3):260-268. doi: 10.1080/15569527.2020.1787435. Epub 2020 Jul 7.
The objective of this study was to investigate the influence of topical preservative-free timolol, benzalkonium chloride(BAC)-preserved timolol, BAC-preserved timolol, and BAC-preserved brimonidine on total protein concentration, advanced oxidation protein products (AOPP) content, total sulfhydryl groups content, the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as Total Oxidant Status (TOS), Total Antioxidant Response, and Oxidative Stress Index (OSI) in the tear film.
The patients were divided into four groups: group C ( = 25)-control group-subjects who did not use topical antiglaucoma medications, group T ( = 17)-patients using topical preservative-free timolol, group T + BAC ( = 24)-patients using topical BAC-preserved timolol, and group BR + BAC ( = 19)-patients using topical BAC-preserved brimonidine.
The SOD, CAT, and GPx activities as well as AOPP, TOS, and OSI were found to be higher in the tear film of patients treated with BAC-preserved topical timolol or brimonidine in comparison with patients treated with preservative-free timolol or patients who did not use antiglaucoma topical medications.
This indicates that using BAC-preserved topical medications increases oxidative stress in the tear film and may, in the long-term, contribute to the clinical presentation of dry eye disease.
本研究旨在探讨局部无防腐剂噻吗洛尔、苯扎氯铵（BAC）保存的噻吗洛尔、BAC 保存的噻吗洛尔和 BAC 保存的溴莫尼定对泪膜总蛋白浓度、晚期氧化蛋白产物（AOPP）含量、总巯基含量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性以及总氧化剂状态（TOS）、总抗氧化反应和氧化应激指数（OSI）的影响。
患者分为四组：C 组（n=25）-对照组-未使用局部降眼压药物的受试者；T 组（n=17）-使用局部无防腐剂噻吗洛尔的患者；T+BAC 组（n=24）-使用局部 BAC 保存的噻吗洛尔的患者；BR+BAC 组（n=19）-使用局部 BAC 保存的溴莫尼定的患者。
与使用无防腐剂局部噻吗洛尔或未使用降眼压局部药物的患者相比，使用 BAC 保存的局部噻吗洛尔或溴莫尼定治疗的患者泪膜中的 SOD、CAT 和 GPx 活性以及 AOPP、TOS 和 OSI 均较高。
这表明使用 BAC 保存的局部药物会增加泪膜中的氧化应激，并可能在长期内导致干眼疾病的临床表现。
