Wheeler A W, Spackman V M, Cottam G P, Moran D M
Bencard Allergy Unit, Beecham Pharmaceuticals, Epsom, UK.
Int Arch Allergy Appl Immunol. 1988;86(1):1-8. doi: 10.1159/000234599.
Rye grass pollen extract was fragmented by sequential treatments with cyanogen bromide and 2-nitro-5-thiocyanobenzoic acid, and a fraction containing fragments of molecular weight greater than 10,000 Mr was isolated. The in vitro reactivity of the extract with specific IgE was extensively reduced by fragmentation. Less reduction in activity was shown either by skin testing or by inhibition of an extract-specific IgG-binding assay. Reactivity with, and ability to induce, extract-specific mouse T cells were retained by the fragment preparation, and the ability to cause transformation of lymphocytes from atopic donors was unchanged. Fragments did not induce extract-specific IgG antibody in mice, were unable to stimulate the production of T-helper cells which could collaborate in an adoptive cell-transfer system, and did not induce delayed hypersensitivity reactions in guinea pigs. The possibility that such T-cell-reactive modified allergens (T'allergoids) might be used to stimulate selectively T-cell subsets and, therefore, could be used to advantage in immunotherapy is discussed.
黑麦草花粉提取物经溴化氰和2-硝基-5-硫氰基苯甲酸顺序处理后被片段化，分离出了一个含有分子量大于10,000 Mr片段的级分。提取物与特异性IgE的体外反应性因片段化而大幅降低。皮肤试验或提取物特异性IgG结合试验的抑制所显示的活性降低较少。片段制剂保留了与提取物特异性小鼠T细胞反应的能力以及诱导这种反应的能力，并且引起特应性供体淋巴细胞转化的能力未变。片段在小鼠中不诱导提取物特异性IgG抗体，无法刺激在过继性细胞转移系统中协同作用的辅助性T细胞的产生，并且在豚鼠中不诱导迟发型超敏反应。讨论了这种T细胞反应性修饰变应原（T类变应原）可能用于选择性刺激T细胞亚群，因此可在免疫治疗中发挥优势的可能性。
