Brown Brandon L, Sandelski Morgan M, Drejet Sarah M, Runge Elizabeth M, Shipchandler Taha Z, Jones Kathryn J, Walker Chandler L
Department of Anatomy, Cell Biology and Physiology Indiana University School of Medicine Indianapolis Indiana USA.
Department of Anatomical Sciences and Neurobiology University of Louisville Louisville Kentucky USA.
Laryngoscope Investig Otolaryngol. 2020 May 28;5(3):552-559. doi: 10.1002/lio2.411. eCollection 2020 Jun.
To determine whether functional and anatomical outcomes following suture neurorrhaphy are improved by the addition of electrical stimulation with or without the addition of polyethylene glycol (PEG).
In a rat model of facial nerve injury, complete facial nerve transection and repair was performed via (a) suture neurorrhaphy alone, (b) neurorrhaphy with the addition of brief (30 minutes) intraoperative electrical stimulation, or (c) neurorrhaphy with the addition electrical stimulation and PEG. Functional recovery was assessed weekly for 16 weeks. At 16 weeks postoperatively, motoneuron survival, amount of regrowth, and specificity of regrowth were assessed by branch labeling and tissue analysis.
The addition of brief intraoperative electrical stimulation improved all functional outcomes compared to suturing alone. The addition of PEG to electrical stimulation impaired this benefit. Motoneuron survival, amount of regrowth, and specificity of regrowth were unaltered at 16 weeks postoperative in all treatment groups.
The addition of brief intraoperative electrical stimulation to neurorrhaphy in this rodent model shows promising neurological benefit in the surgical repair of facial nerve injury.
Animal study.
确定在有或没有添加聚乙二醇（PEG）的情况下，增加电刺激是否能改善缝合神经吻合术后的功能和解剖学结果。
在大鼠面神经损伤模型中，通过以下方式进行完全性面神经横断和修复：（a）仅行缝合神经吻合术；（b）在神经吻合术基础上增加短暂（30分钟）的术中电刺激；或（c）在神经吻合术基础上增加电刺激和PEG。每周评估功能恢复情况，持续16周。术后16周，通过分支标记和组织分析评估运动神经元存活、再生量和再生特异性。
与单纯缝合相比，增加短暂术中电刺激可改善所有功能结果。在电刺激基础上添加PEG会削弱这种益处。所有治疗组术后16周时运动神经元存活、再生量和再生特异性均未改变。
在该啮齿动物模型中，在神经吻合术中增加短暂术中电刺激在面神经损伤的手术修复中显示出有前景的神经学益处。
动物研究。
