Department of Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), University of Science and Technology of China, Hefei, 230026, China.
National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, 230026, China.
Small. 2020 Aug;16(31):e2002343. doi: 10.1002/smll.202002343. Epub 2020 Jun 29.
Nanomaterials with enzyme-mimicking characteristics have engaged great awareness in various fields owing to their comparative low cost, high stability, and large-scale preparation. However, the wide application of nanozymes is seriously restricted by the relatively low catalytic activity and poor specificity, primarily because of the inhomogeneous catalytic sites and unclear catalytic mechanisms. Herein, a support-sacrificed strategy is demonstrated to prepare a single iron site nanozyme (Fe SSN) dispersed on the porous N-doped carbon. With well-defined coordination structure and high density of active sites, the Fe SSN performs prominent peroxidase-like activity by efficiently activating H O into hydroxyl radical (•OH) species. Furthermore, the Fe SSN is applied in colorimetric detection of glucose through a multienzyme biocatalytic cascade platform. Moreover, a low-cost integrated agarose-based hydrogel colorimetric biosensor is designed and successfully achieves the visualization evaluation and quantitative detection of glucose. This work expands the application of single-site catalysts in the fields of nanozyme-based biosensors and personal biomedical diagnosis.
具有酶模拟特性的纳米材料由于其成本相对较低、稳定性高和大规模制备等特点，在各个领域引起了极大的关注。然而，纳米酶的广泛应用受到其催化活性相对较低和特异性较差的严重限制，主要是由于其催化位点不均匀和催化机制不明确。本文展示了一种通过牺牲载体的策略来制备单铁位纳米酶（Fe SSN），该纳米酶分散在多孔氮掺杂碳上。由于具有明确的配位结构和高密度的活性位点，Fe SSN 通过高效地将 H O 激活为羟基自由基（•OH）物种，表现出突出的过氧化物酶样活性。此外，Fe SSN 通过多酶生物催化级联平台应用于葡萄糖的比色检测。此外，设计了一种低成本的基于琼脂糖的水凝胶比色生物传感器，并成功实现了葡萄糖的可视化评估和定量检测。这项工作扩展了单原子催化剂在基于纳米酶的生物传感器和个人生物医学诊断领域的应用。
