Liu Wene, Tian Lili, Du Jie, Wu Jiangmin, Liu Yongmei, Wu Guofan, Lu Xiaoquan
College of Life Sciences, Northwest Normal University, Lanzhou 730070, Gansu, China.
Analyst. 2020 Aug 21;145(16):5500-5507. doi: 10.1039/d0an00930j. Epub 2020 Jun 29.
Developing a rapid, low-cost, and multimode detection method for heavy metal ions remains a compelling goal for many applications, including food safety, environmental and biological analysis. This study investigated the influence of Hg on the peroxidase-like activity of gold nanoparticles (GNPs) decorated on carbon dots (CDs) from lysine (denoted as GNP@CDs). A new type of Hg-triggered peroxidase-like activity of GNP@CDs was discovered, which could catalyze the oxidation of the colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue TMB. Based on the regulation of the catalytically triggered activity, a sensitive colorimetric method for the detection of Hg was developed, with a linear range of 7-150 nM, providing a limit of detection as low as 3.7 nM. The sensor is simple and rapid, and was successfully applied to the detection of Hg enrichment in chlorella, suggesting a promising application in biological analysis.
开发一种快速、低成本且多模式的重金属离子检测方法，对于包括食品安全、环境和生物分析在内的许多应用而言，仍然是一个极具吸引力的目标。本研究考察了汞对赖氨酸修饰的碳点（CDs）负载的金纳米颗粒（GNPs，记为GNP@CDs）类过氧化物酶活性的影响。发现了一种新型的汞触发GNP@CDs类过氧化物酶活性，其可催化无色的3,3',5,5'-四甲基联苯胺（TMB）氧化为蓝色TMB。基于对催化触发活性的调控，开发了一种灵敏的比色法用于检测汞，线性范围为7-150 nM，检测限低至3.7 nM。该传感器简单快速，并成功应用于小球藻中汞富集的检测，表明其在生物分析中具有广阔的应用前景。
