College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, China.
College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing 400715, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Mar 5;268:120658. doi: 10.1016/j.saa.2021.120658. Epub 2021 Nov 24.
Adenosine triphosphate (ATP) is the main energy currency for cells and an important biomolecule involved in cellular reactions, whose abnormal levels are closely related to physical disease, thus it is extremely important to establish a convenient, fast and simple ATP monitoring method. Toward this end, we developed a facile method for colorimetric detection of ATP on the basis of the inhibiting effect of ATP on the peroxidase-like activity of carbon dots (CDs). The detection principle of this method was utilizing the peroxidase-like activity of CDs, which catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HO to generate blue products. However, the introduction of ATP in the system can inhibit the generation of blue products, so ATP can be colorimetric detected. This method exhibited high sensitivity with a detection limit of 34 nM and a wide linear range (0.050-2.0 μM). The as-proposed colorimetric ATP sensor was capable of detecting ATP in real samples accurately.
三磷酸腺苷（ATP）是细胞的主要能量货币，也是参与细胞反应的重要生物分子，其异常水平与物理疾病密切相关，因此建立一种方便、快速、简单的 ATP 监测方法非常重要。为此，我们基于 ATP 对碳点（CDs）过氧化物酶样活性的抑制作用，开发了一种用于 ATP 比色检测的简便方法。该方法的检测原理是利用 CDs 的过氧化物酶样活性，催化 HO 将 3,3',5,5'-四甲基联苯胺（TMB）氧化生成蓝色产物。然而，体系中加入的 ATP 可以抑制蓝色产物的生成，因此可以对 ATP 进行比色检测。该方法具有较高的灵敏度，检测限为 34 nM，线性范围较宽（0.050-2.0 μM）。所提出的比色 ATP 传感器能够准确地检测真实样品中的 ATP。
