School of Industrial and Systems Engineering, Georgia Institute of Technology, Atlanta, Georgia.
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia.
Biotechnol Bioeng. 2020 Oct;117(10):3081-3093. doi: 10.1002/bit.27480. Epub 2020 Jul 14.
This study describes the response of Arthrospira platensis to a variety of temperature conditions as reflected in variations of photosynthetic parameters, pigmentation, and biomass productivity in indoor photobioreactor (PBR) cultivations. These experiments are designed to better understand the impact of temperature, seasonal variations, and acclimation effects on outdoor biomass production. The irradiance level and temperature range (20-39°C) are chosen to enable modeling of semi-continuous operation of large-scale outdoor PBR deployments. Overall, the cultivations are quite stable with some pigment-related instabilities after prolonged high-temperature exposure. Changes in productivity with temperature, as reflected in measured photosynthetic parameters, are immediate and mainly attributable to the temperature dependence of the photosaturation parameter, a secondary factor being variation in pigment content on a longer time scale corresponding to turnover of the culture population. Though pigment changes are not accompanied by significant changes in productivity, prolonged exposure at 35°C and above yields a clear degradation in performance. Productivities in a semi-continuous operation are quantitatively reproduced with a productivity model incorporating photosynthetic parameters measured herein. This study confirms the importance of temperature for biomass and pigment production in Arthrospira cultivations and provides a basis for risk assessments related to temperature mitigation for large-scale outdoor cultivations.
本研究描述了节旋藻对各种温度条件的响应，反映在室内光生物反应器 (PBR) 培养中光合作用参数、色素和生物量生产力的变化。这些实验旨在更好地了解温度、季节性变化和驯化效应对室外生物量生产的影响。选择辐照度水平和温度范围（20-39°C）是为了能够对大规模室外 PBR 部署的半连续运行进行建模。总的来说，培养物非常稳定，长时间高温暴露后会出现一些与色素有关的不稳定性。光合作用参数测量反映出的生产力随温度的变化是即时的，主要归因于光饱和参数的温度依赖性，次要因素是色素含量的变化，这与文化群体的更替有关，时间尺度较长。尽管色素变化并不伴随着生产力的显著变化，但在 35°C 及以上长时间暴露会导致性能明显下降。用本研究中测量的光合作用参数的生产力模型定量再现了半连续操作的生产力。本研究证实了温度对节旋藻培养物生物量和色素生产的重要性，并为与大规模室外培养物的温度缓解相关的风险评估提供了依据。
