Unit of Human Microbiome, Bambino Gesù Children's Hospital IRCCS, Piazza Sant'Onofrio 4, 00165 Rome, Italy.
Unit of Parasitology, Bambino Gesù Children's Hospital IRCCS, Piazza Sant'Onofrio 4, 00165 Rome, Italy.
Genes (Basel). 2020 Jun 24;11(6):693. doi: 10.3390/genes11060693.
Anisakiasis is nowadays a well-known infection, mainly caused by the accidental ingestion of larvae, following the consumption of raw or undercooked fishes and cephalopods. Due to the similarity of symptoms with those of common gastrointestinal disorders, this infection is often underestimated, and the need for new specific diagnostic tools is becoming crucial. Given the remarkable impact that MALDI-TOF MS biotyping had in the last decade in clinical routine practice for the recognition of bacterial and fungi strains, a similar scenario could be foreseen for the identification of parasites, such as nematodes. In this work, a MALDI-TOF MS profiling of proteome was pursued with a view to constructing a first spectral library for the diagnosis of infections. At the same time, a shotgun proteomics approach by LC-ESI-MS/MS was performed on the two main fractions obtained from protein extraction, to evaluate the protein species enriched by the protocol. A set of MALDI-TOF MS signals associated with proteins originating in the ribosomal fraction of the nematode extract was selected as a potential diagnostic tool for the identification of spp.
现如今，旋毛虫病是一种广为人知的感染，主要是由于人们食用了生的或未煮熟的鱼类和头足类动物，意外摄入了幼虫。由于这种感染的症状与常见的胃肠道疾病相似，因此常常被低估，因此，对新的特异性诊断工具的需求变得至关重要。鉴于基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）生物分型在过去十年中对细菌和真菌菌株的临床常规实践中的识别具有显著影响，可以预见类似的情况也将出现在寄生虫（如线虫）的鉴定中。在这项工作中，我们对蛋白质组进行了 MALDI-TOF MS 分析，以期构建用于诊断旋毛虫感染的第一个光谱库。同时，我们还通过 LC-ESI-MS/MS 对从蛋白质提取中获得的两个主要部分进行了鸟枪法蛋白质组学分析，以评估该方案中富集的蛋白质种类。从线虫提取物的核糖体部分中选择了一组与蛋白质相关的 MALDI-TOF MS 信号，作为鉴定旋毛虫属 spp.的潜在诊断工具。
