Kim Jeong-Ho, Kim Jong-Oh, Jeon Chan-Hyeok, Nam U-Hwa, Subramaniyam Sathiyamoorthy, Yoo Seung-Il, Park Jun-Hyung
Department of Marine Bioscience, Gangneung-Wonju National University, Gangneung, 25457, Republic of Korea.
Institute of Marine Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.
Mol Biochem Parasitol. 2018 Dec;226:24-33. doi: 10.1016/j.molbiopara.2018.10.002. Epub 2018 Oct 26.
We analyzed transcriptome profiles of Anisakis simplex (Nematoda: Anisakidae) 3rd (ASL3) and 4th larvae (ASL4) obtained by RNA-seq, to understand the molecular pathways linked to parasite survival and discover stage-enriched gene expressions. ASL3 were collected from chum salmon and ASL4 were obtained by in vitro culture. Whole transcriptome sequencing was conducted with Illumina sequencer, and de novo assembly was conducted. 47,179 and 41,934 genes were expressed in ASL3 and ASL4 transcriptomes. Of them, 17,633 were known and 29,546 were unmapped sequence for ASL3. 17,126 were known and 24,808 were unmapped sequence for ASL4. Polyubiquitins-related genes and collagen-related genes were the most abundantly expressed in ASL3 and ASL4. Mitochondrial enzyme-related genes were highly expressed both in ASL3 and ASL4. Among the transcripts, 675 were up-regulated in ASL3, while 1015 were up-regulated in ASL4. Several protease-related and protein biosynthesis-related genes were highly expressed in ASL3, all of which are thought to be crucial for invading host tissues. Collagen synthesis-related genes were highly expressed in ASL4, reflecting active biosynthesis of collagens during molting process. This information will extend our understanding of biology of the fish-borne zoonotic parasite A. simplex.
我们分析了通过RNA测序获得的简单异尖线虫（线虫纲：异尖科）第三期幼虫（ASL3）和第四期幼虫（ASL4）的转录组图谱，以了解与寄生虫存活相关的分子途径，并发现阶段富集的基因表达。ASL3从大麻哈鱼中收集，ASL4通过体外培养获得。使用Illumina测序仪进行全转录组测序，并进行从头组装。在ASL3和ASL4转录组中分别表达了47179个和41934个基因。其中，ASL3中有17633个是已知基因，29546个是未映射序列。ASL4中有17126个是已知基因，24808个是未映射序列。多聚泛素相关基因和胶原蛋白相关基因在ASL3和ASL4中表达最为丰富。线粒体酶相关基因在ASL3和ASL4中均高表达。在这些转录本中，675个在ASL3中上调，而1015个在ASL4中上调。几个蛋白酶相关基因和蛋白质生物合成相关基因在ASL3中高表达，所有这些基因都被认为对侵入宿主组织至关重要。胶原蛋白合成相关基因在ASL4中高表达，反映了蜕皮过程中胶原蛋白的活跃生物合成。这些信息将扩展我们对鱼传人畜共患寄生虫简单异尖线虫生物学的理解。
