School of Materials Science and Engineering, Key Lab. for Anisotropy and Texture of Materials, Education Ministry of China, Northeastern University, Shenyang 110819, China.
School of Metallurgy, Northeastern University, Shenyang 110819, China.
Mater Sci Eng C Mater Biol Appl. 2020 Oct;115:110921. doi: 10.1016/j.msec.2020.110921. Epub 2020 Apr 11.
In order to improve antibacterial properties and cell biocompatibility of Ti-Cu alloy, an ultrasonic micro-arc oxidation (UMAO) has been applied to Ti-Cu alloy. The corrosion resistance, antibacterial activity and cell compatibility of Ti-Cu alloy before and after UMAO were studied in detail by means of electrochemical test, plate count method and CCK-8 test scanning electron microscopy (SEM) technology to evaluate the application possibilities of UMAO as a surface bio-modification method for Ti-Cu alloy. The surface microstructure characterisation showed that a typical porous coating with a pore diameter of 3-8 μm and a thickness of 5-15 μm was formed on the surface of the Ti-Cu alloy, which significantly improved the surface roughness and hydrophilicity. The plate count method demonstrated that UMAO coatings on Ti-Cu alloy showed strong antibacterial activity (≥99%) against Staphylococcus aureus (S. aureus) even after being immersed in a physiological saline for up to 20 days, indicating that UMAO-treated Ti-Cu alloy had very strong long-term antibacterial properties. It is believed that the strong long-term antimicrobial properties of Ti-Cu-UMAO samples were mainly due to the formation of CuO and CuO in UMAO coatings. The results of cell compatibility evaluation showed that UMAO treatment did not bring about cytotoxicity but improved the early adhesion of MC3T3 cell.
为了提高 Ti-Cu 合金的抗菌性能和细胞生物相容性，采用超声微弧氧化（UMAO）技术对 Ti-Cu 合金进行表面处理。通过电化学测试、平板计数法和 CCK-8 试验，详细研究了 UMAO 前后 Ti-Cu 合金的耐腐蚀性、抗菌活性和细胞相容性，采用扫描电子显微镜（SEM）技术对 UMAO 作为 Ti-Cu 合金表面生物改性方法的应用可能性进行了评价。表面微观结构分析表明，Ti-Cu 合金表面形成了一种典型的多孔氧化层，孔径为 3-8μm，厚度为 5-15μm，显著提高了表面粗糙度和润湿性。平板计数法表明，UMAO 处理后的 Ti-Cu 合金对金黄色葡萄球菌（S. aureus）具有很强的抗菌活性（≥99%），即使在生理盐水中浸泡长达 20 天，这表明 UMAO 处理的 Ti-Cu 合金具有很强的长期抗菌性能。据信，Ti-Cu-UMAO 样品具有很强的长期抗菌性能主要是由于 UMAO 涂层中形成了 CuO 和 CuO。细胞相容性评价结果表明，UMAO 处理不会带来细胞毒性，反而会提高 MC3T3 细胞的早期黏附性。
