Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego 6 Street, 44-100 Gliwice, Poland.
Faculty of Mechanical Engineering, Koszalin University of Technology, Racławicka 15-17, 75-620 Koszalin, Poland.
Mater Sci Eng C Mater Biol Appl. 2020 Oct;115:111098. doi: 10.1016/j.msec.2020.111098. Epub 2020 May 20.
This paper reports on the plasma electrolytic oxidation (PEO) of titanium alloy Ti-15Mo in baths containing zinc to obtain biomaterials with bacteriostatic and antibacterial properties. The Ti-15Mo surface was oxidised in a 0.1 M Ca(HPO) bath containing zinc compound particles: ZnO or Zn(PO). During the PEO process, the applied voltage was 300 V, and the current density was 150 mA∙cm. The surface morphology, roughness and wettability were determined. It has been noted that both roughness and wettability of Ti-15Mo alloy surface increased after PEO. EDX and XPS chemical composition analysis was carried out, and Raman spectroscopy was also performed indicating that Zn has been successfully incorporated into oxide layer. To investigate the antibacterial properties of the PEO oxide coatings, microbial tests were carried out. The bacterial adhesion test was performed using four different bacterial strains: reference Staphylococcus aureus (ATCC 25923), clinical Staphylococcus aureus (MRSA 1030), reference Staphylococcus epidermidis (ATCC 700296) and clinical Staphylococcus epidermidis (15560). Performed zinc-containing oxide coatings did not indicate the bacteria growth inducing effect. Additionally, the cytocompatibility of the formed oxide layers was characterised by MG-63 osteoblast-like live/dead tests. The surface bioactivity and cytocompatibility increased after the PEO process. The zinc was successfully incorporated into the titanium oxide layer. Based on the obtained results of the studies, it can be claimed that zinc-containing PEO layers can be an interesting course of bacteriostatic titanium biomaterials development.
本文报告了在含有锌的浴中对钛合金 Ti-15Mo 进行等离子电解氧化 (PEO)，以获得具有抑菌和抗菌性能的生物材料。Ti-15Mo 表面在含有锌化合物颗粒（氧化锌或磷酸锌）的 0.1 M Ca(HPO)浴中被氧化。在 PEO 过程中，施加的电压为 300 V，电流密度为 150 mA·cm。测定了表面形貌、粗糙度和润湿性。结果表明，PEO 后 Ti-15Mo 合金表面的粗糙度和润湿性都有所提高。进行了 EDX 和 XPS 化学成分分析，还进行了拉曼光谱分析，表明 Zn 已成功掺入氧化物层中。为了研究 PEO 氧化物涂层的抗菌性能，进行了微生物测试。使用四种不同的细菌菌株进行了细菌粘附测试：参考金黄色葡萄球菌（ATCC 25923）、临床金黄色葡萄球菌（MRSA 1030）、参考表皮葡萄球菌（ATCC 700296）和临床表皮葡萄球菌（15560）。结果表明，含锌的氧化物涂层没有表现出诱导细菌生长的效果。此外，还通过 MG-63 成骨样细胞死活试验对形成的氧化物层的细胞相容性进行了表征。PEO 处理后，表面生物活性和细胞相容性增加。锌已成功掺入钛氧化物层中。基于所研究结果，可以声称含锌的 PEO 层可以成为具有抑菌性的钛生物材料开发的一个有趣途径。
