Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine, 310 Cedar street, LSOG 304B, New Haven CT, 06525, USA.
Foundation for Embryonic Eompetence, 140 Allen Road, Basking Ridge NJ, 07920, USA.
Reprod Biomed Online. 2020 Aug;41(2):183-190. doi: 10.1016/j.rbmo.2020.04.011. Epub 2020 May 14.
Does mitochondrial DNA (mtDNA) copy number predict the reproductive potential of euploid human blastocysts?
To investigate whether the amount of mtDNA in trophectoderm biopsies correlates with IVF outcome, euploid human blastocysts (n = 615) used in single embryo transfer were analysed. Furthermore, to determine whether mtDNA content is predictive of reproductive outcome within a given cohort, paired sibling embryos (n = 78) transferred in two consecutive cycles carried out in the same patient (in which one cycle failed to result in implantation and the other cycle resulted in sustained implantation) were studied. Targeted amplification followed by quantitative real-time polymerase chain reaction for two mitochondrial loci (16S and MajArc) relative to a multicopy nuclear genome locus (AluYb8) were carried out to determine relative mtDNA copy number.
Sustained implantation was not associated with relative mtDNA copy number (P = 0.78), and there was no threshold value above or below which ongoing implantation was more or less likely. No correlation was observed between maternal age and relative mtDNA copy number (P = 0.39). In addition, no association was found between relative mtDNA levels of sibling embryos and ensuing implantation and delivery rates in women who underwent a successful single embryo transfer before or after a failed transfer using embryos derived from the same cohort of oocytes (P = 0.70).
In trophectoderm samples, mitochondrial DNA copy number analysis was not found to be predictive of euploid human embryo reproductive competence. These data do not support the use of mitochondrial DNA copy number in clinical decision making when selecting which embryo to transfer.
线粒体 DNA（mtDNA）拷贝数是否可预测整倍体人类囊胚的生殖潜能？
为了研究滋养外胚层活检中的 mtDNA 量是否与 IVF 结局相关，分析了用于单胚胎移植的整倍体人类囊胚（n=615）。此外，为了确定 mtDNA 含量是否可预测特定队列中的生殖结局，研究了在同一患者中连续两个周期（一个周期未导致着床，另一个周期导致持续着床）移植的配对同卵双胞胎胚胎（n=78）。通过靶向扩增，然后对两个线粒体基因座（16S 和 MajArc）相对于多拷贝核基因组基因座（AluYb8）进行定量实时聚合酶链反应，以确定相对 mtDNA 拷贝数。
持续着床与相对 mtDNA 拷贝数无关（P=0.78），并且不存在持续着床更有可能或更不可能的上下阈值。母体年龄与相对 mtDNA 拷贝数之间没有相关性（P=0.39）。此外，在经历过一次成功的单胚胎移植的女性中，无论使用来自同一卵母细胞队列的胚胎在前一次移植失败后进行移植还是在该次移植前进行移植，同卵双胞胎胚胎的相对 mtDNA 水平与随后的着床和分娩率之间均未发现相关性（P=0.70）。
在滋养外胚层样本中，未发现线粒体 DNA 拷贝数分析可预测整倍体人类胚胎的生殖能力。这些数据不支持在选择要移植的胚胎时，将线粒体 DNA 拷贝数用于临床决策。
