Department of Pharmaceutical Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan.
Department of Pharmacology and Medical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan.
Acta Diabetol. 2020 Nov;57(11):1351-1358. doi: 10.1007/s00592-020-01562-0. Epub 2020 Jun 29.
The study evaluated the effect of different concentrations of sildenafil-containing ointment on wound healing in healthy and streptozotocin (STZ)-induced diabetic rats.
A total of 108 Sprague Dawley male rats aged 5 months were randomly divided into two groups: healthy and diabetes-inducing rats. Following induction of diabetes by intraperitoneal STZ injection (55 mg/kg), diabetic and healthy rats were subdivided into six groups (9 rats each). Linear incisions of the dorsal backs of rats were made. Then, rats were treated twice daily with either 2%, 2% + Fucidin, 5% sildenafil-containing ointments, oral sildenafil (13.0 mg/kg), moist exposed burn ointment or vehicle. During treatment, the percent reduction of the wound area and tensile strength were measured on days 3, 7 and 10. Furthermore, histopathology was performed on the wounded skin on similar days for the assessment of collagen synthesis and proliferation of new capillary vessels.
In healthy and STZ-induced diabetic rats, the percent reduction in wound area on day 3 was significantly higher in sildenafil-containing ointment-treated groups than all other groups, whereas on day 7 only the 5% sildenafil-containing ointment-treated group showed better response in healthy rats. Although the sildenafil-containing ointment significantly showed better tensile strength than all other groups, the 5% sildenafil-treated group significantly enhanced the tensile better than the 2% and 2% + Fucidin dose. Furthermore, the histological evaluation revealed that sildenafil-containing ointment promoted collagen synthesis and proliferation of new capillary vessels.
Our results suggest that sildenafil-containing ointment can provide an advantage in wound healing by promoting wound contractions and resistance to wound breakage in healthy and diabetic conditions. Therefore, 5% sildenafil-containing ointment can be used as a support factor for wound healing in healthy and diabetic conditions; however, clinical trials are required to confirm the benefits of sildenafil in wound healing.
本研究评估了不同浓度含有西地那非的软膏对健康和链脲佐菌素（STZ）诱导的糖尿病大鼠伤口愈合的影响。
将 108 只 5 月龄雄性 Sprague Dawley 大鼠随机分为两组：健康组和糖尿病诱导组。通过腹腔注射 STZ（55mg/kg）诱导糖尿病后，糖尿病和健康大鼠进一步分为六组（每组 9 只）。在大鼠背部做线性切口。然后，大鼠每天两次接受 2%、2%+Fucidin、5%含有西地那非的软膏、口服西地那非（13.0mg/kg）、湿润烧伤膏或赋形剂治疗。在治疗过程中，于第 3、7 和 10 天测量伤口面积减少百分比和拉伸强度。此外，在相似的日子对受伤皮肤进行组织病理学检查，以评估胶原合成和新毛细血管的增殖。
在健康和 STZ 诱导的糖尿病大鼠中，与其他所有组相比，含有西地那非的软膏治疗组在第 3 天的伤口面积减少百分比显著更高，而在第 7 天，只有 5%含有西地那非的软膏治疗组在健康大鼠中显示出更好的反应。虽然含有西地那非的软膏显著显示出比所有其他组更好的拉伸强度，但 5%西地那非治疗组显著优于 2%和 2%+Fucidin 剂量增强拉伸强度。此外，组织学评估显示，含有西地那非的软膏促进了胶原合成和新毛细血管的增殖。
我们的结果表明，含有西地那非的软膏可通过促进伤口收缩和提高健康和糖尿病状态下伤口破裂的抵抗力，为伤口愈合提供优势。因此，5%含有西地那非的软膏可作为健康和糖尿病状态下伤口愈合的支持因素；然而，需要临床试验来确认西地那非在伤口愈合中的益处。
