Department of Biological Sciences, Birla Institute of Technology and Science (BITS), Pilani, Rajasthan, India.
Department of Pharmacy, Birla Institute of Technology and Science (BITS), Pilani, Rajasthan, India.
Int J Phytoremediation. 2020;22(14):1487-1496. doi: 10.1080/15226514.2020.1784088. Epub 2020 Jun 30.
This study focused on isolation of bacteria with biphenyl/polychlorinated biphenyl (PCB) degrading ability from the rhizosphere of (mulberry plant). Repetitive enrichment of rhizospheric soil samples with biphenyl resulted in the isolation of sp. MAPN-1, identified by 16S rRNA gene sequence analysis. The bacterium showed growth on five different aromatic compounds (naphthalene, salicylic acid, benzoic acid, dibenzofuran and anthracene). Benzoic acid was detected as the major metabolite during biphenyl degradation using high-performance thin-layer chromatography (HPTLC) with R 0.42 at 254 nm. Further GC-MS/MS study showed 95% and 15% degradation of biphenyl and dichlorobiphenyl, respectively. A pot study was conducted to evaluate the effect of presence of biphenyl on and the role of biphenyl degrader sp. MAPN-1 in relation to phytoremediation. twigs in biphenyl spiked soil (100 mg/kg and 300 mg/kg) inoculated with sp. MAPN-1 showed growth, whereas, growth of plants (control) was adversely affected in biphenyl-spiked uninoculated soil. It is the first report of isolation of sp. MAPN-1 from the rhizosphere of , its capability to degrade biphenyl, thereby showing a positive effect on the plant growth grown in biphenyl spiked soil.
本研究从 （桑树植物）的根际土壤中分离出具有联苯/多氯联苯（PCB）降解能力的细菌。通过重复富集根际土壤样品中的联苯，分离出 sp. MAPN-1，通过 16S rRNA 基因序列分析进行鉴定。该细菌在 5 种不同的芳香族化合物（萘、水杨酸、苯甲酸、二苯并呋喃和蒽）上表现出生长。使用高效薄层层析（HPTLC）并用 254nm 处的 R 0.42 检测到联苯降解过程中苯甲酸为主要代谢物。进一步的 GC-MS/MS 研究表明，联苯和二氯联苯分别降解了 95%和 15%。进行了盆栽研究以评估存在联苯对 和联苯降解菌 sp. MAPN-1 与植物修复之间关系的影响。在接种了 sp. MAPN-1 的联苯污染土壤（100mg/kg 和 300mg/kg）中的 嫩枝显示出生长，而在未接种的联苯污染土壤中，对照植物（对照）的生长受到不利影响。这是首次从 的根际土壤中分离出 sp. MAPN-1 的报道，它能够降解联苯，从而对生长在联苯污染土壤中的植物生长产生积极影响。
