School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550002, China.
Dazhou Integrated, TCM&Western Medicine Hospital, Dazhou 635000, China.
J Asian Nat Prod Res. 2021 Aug;23(8):764-771. doi: 10.1080/10286020.2020.1786065. Epub 2020 Jun 30.
Three compounds with diuretic potential were identified from the 95% ethanol extract of (Christ) Ching. Among them, one was a new benzanilide named petiolide A (), and the other two were phenolic derivatives barbatic acid () and kaempferol (). Their structures were elucidated based on extensive spectral analyses and comparison with the literature data. The docking experiments of all compounds into the active site of the With-No-Lysine kinase 1 (WNK1) domain demonstrated that kaempferol () was the most effective component with diuretic potential for its comparative diuretic effect to that of an orally bioavailable WNK inhibitor WNK463 (docking score -10.99 vs -11.09).[Formula: see text].
从（Christ）Ching 的 95%乙醇提取物中鉴定出三种具有利尿作用的化合物。其中一种是新型苯甲酰胺类化合物，名为 petiolide A（），另外两种是酚类衍生物巴豆酸（）和山柰酚（）。基于广泛的光谱分析并与文献数据进行比较，确定了它们的结构。所有化合物与无赖氨酸激酶 1（WNK1）结构域的对接实验表明，山柰酚（）是最有效的利尿作用成分，其利尿作用与口服生物利用度的 WNK 抑制剂 WNK463（对接评分-10.99 对-11.09）相当。
