理想的媒介传播疾病的精确小鼠模型标准，重点关注丛林型斑疹伤寒和登革热。
Ideal Criteria for Accurate Mouse Models of Vector-Borne Diseases with Emphasis on Scrub Typhus and Dengue.
机构信息
1Department of Pathology, Sealy Institute for Vaccine Sciences, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas.
2Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas.
出版信息
Am J Trop Med Hyg. 2020 Sep;103(3):970-975. doi: 10.4269/ajtmh.19-0955.
Nine criteria regarding the infectious agent, mode of transmission, portal of entry, route of spread, target organs, target cells, pathologic lesions, incubation period, and modifiable spectrum of disease and outcomes appropriate to the intended experimental purpose are described. To provide context for each criterion, mouse models of two vector-borne zoonotic infectious diseases, scrub typhus and dengue, are summarized. Application of the criteria indicates that intravenous inoculation of into inbred mice is the best current model for life-threatening scrub typhus, and intradermal inoculation accurately models sublethal human scrub typhus, whereas the immunocompromised mouse models of dengue provide disease outcomes most closely associated with human dengue. In addition to addressing basic questions of immune and pathogenic mechanisms, mouse models are useful for preclinical testing of experimental vaccines and therapeutics. The nine criteria serve as guidelines to evaluate and compare models of vector-borne infectious diseases.
描述了九个与感染因子、传播方式、入口门户、传播途径、靶器官、靶细胞、病理损伤、潜伏期以及针对特定实验目的的可改变疾病谱和结局相关的标准。为了给每个标准提供背景，总结了两种虫媒动物传染病（恙虫病和登革热）的小鼠模型。应用这些标准表明，将 静脉内接种于近交系小鼠是目前威胁生命的恙虫病的最佳模型，而皮内接种准确地模拟了亚致死性人类恙虫病，而免疫功能低下的登革热小鼠模型提供了与人类登革热最相关的疾病结局。除了解决免疫和发病机制的基本问题外，小鼠模型还可用于实验疫苗和疗法的临床前测试。这九个标准可作为评估和比较虫媒传染病模型的指南。
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