Van Alstyne D
Am J Clin Pathol. 1977 Jul;68(1):24-8. doi: 10.1093/ajcp/68.1.24.
The extensive use of the single technic of immunofluorescence has resulted in the detection of an array of melanoma-specific antigens. Those antigens residing in the cytoplasm of the melanoma cell have been shown to cross-react across various melanoma cell lines in tissue culture, as well as across all fresh tumor cells isolated directly from patients. The ubiquitous nature of this antigenic activity, as defined by immunofluorescence, suggests that the presence of this activity may be important diagnostically. Isolation of one or more antigens from the cytoplasm, however, requires the development of an assay for antigenic activity in cell fractions. Data presented here indicate that passive hemagglutination provides as effective a means of visualizing antigenic activity as does immunofluorescence. Evidence showing that melanoma-specific antigenic activity is present as a soluble component in the cytosol and as a membrane-associated component of the endoplasmic reticulum is also presented.
免疫荧光单一技术的广泛应用已导致一系列黑色素瘤特异性抗原的检测。已表明存在于黑色素瘤细胞质中的那些抗原在组织培养中能在各种黑色素瘤细胞系之间发生交叉反应，并且在直接从患者分离的所有新鲜肿瘤细胞之间也能发生交叉反应。通过免疫荧光所定义的这种抗原活性的普遍存在性表明，这种活性的存在在诊断上可能很重要。然而，从细胞质中分离一种或多种抗原需要开发一种用于检测细胞组分中抗原活性的测定方法。此处给出的数据表明，被动血凝反应与免疫荧光一样，是可视化抗原活性的有效手段。还给出了证据表明黑色素瘤特异性抗原活性作为一种可溶性成分存在于细胞质溶胶中，并且作为内质网的膜相关成分存在。
