Maccani J E
Am J Clin Pathol. 1977 Jul;68(1):39-44. doi: 10.1093/ajcp/68.1.39.
Counterimmunoelectrophoresis was used to detect Cryptococcus neoformans capsular polysaccharide in the body fluids of infected patients. Using rabbit anti-C. neoformans A15 globulin, counterimmunoelectrophoresis detected 1.25 microgram of purified cryptococcal polysaccharide per ml of cerebrospinal fluid (CSF) or urine and 2.5 microgram per ml of serum. When the body fluids were subjected to a hundredfold concentration prior to testing, as little as 25 ng of purified cryptococcal polysaccharide/ml CSF or urine and 80 ng/ml serum was detected. Among patients with cryptococcal meningitis, counterimmunoelectrophoresis detected polysaccharide in the CSF when the India-ink examination was negative. Using counterimmunoelectrophoresis, the concentrations of polysaccharide antigen in the body fluids of these patients could be estimated. Specificity of the rabbit anti-C. neoformans A15 globulin was evaluated. There was no crossreaction that detracted from its diagnostic usefulness. Rheumatoid factor was not a source of false-positive tests. Counterimmunoelectrophoresis is a useful technic for establishing a rapid, specific serologic diagnosis of cryptococcosis.
采用对流免疫电泳法检测感染患者体液中的新型隐球菌荚膜多糖。使用兔抗新型隐球菌A15球蛋白，对流免疫电泳法检测到每毫升脑脊液（CSF）或尿液中含有1.25微克纯化的隐球菌多糖，血清中每毫升含有2.5微克。在检测前将体液进行100倍浓缩时，每毫升脑脊液或尿液中可检测到低至25纳克的纯化隐球菌多糖，血清中每毫升可检测到80纳克。在新型隐球菌性脑膜炎患者中，当墨汁涂片检查为阴性时，对流免疫电泳法可在脑脊液中检测到多糖。使用对流免疫电泳法，可以估算这些患者体液中多糖抗原的浓度。对兔抗新型隐球菌A15球蛋白的特异性进行了评估。没有交叉反应影响其诊断效用。类风湿因子不是假阳性检测的来源。对流免疫电泳是一种用于快速、特异性血清学诊断隐球菌病的有用技术。
