用于检测和定量新型隐球菌抗原的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen.
作者信息
Illnait M T, Vilaseca J C, Fernández C M, Martínez G F
机构信息
Laboratorio de Micología, Instituto Pedro Kourí, Havana, Cuba.
出版信息
Mem Inst Oswaldo Cruz. 2001 Feb;96(2):241-5. doi: 10.1590/s0074-02762001000200018.
An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.
一种酶联免疫吸附测定法被标准化用于检测血清和脑脊液中的隐球菌抗原。该系统在来自感染人类免疫缺陷病毒且有或无既往隐球菌病诊断的患者的临床样本中进行了评估。所评估的系统具有高度敏感性和特异性，并且与乳胶凝集法相比无显著差异。用纯化的新型隐球菌抗原建立了标准曲线，用于对阳性样本中的抗原进行定量。
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