用[3H]R1881对正常个体和雄激素受体异常患者的人培养生殖成纤维细胞中的雄激素受体进行光亲和标记。

Photoaffinity labelling with [3H]R1881 of androgen receptors from human cultured genital fibroblasts of normal individuals and patients with androgen receptor abnormalities.

作者信息

Gyorki S, Warne G L, Funder J W

机构信息

Medical Research Centre, Prince Henry's Hospital, Melbourne, Australia.

出版信息

J Steroid Biochem. 1988 Jun;29(6):611-5. doi: 10.1016/0022-4731(88)90159-8.

DOI:10.1016/0022-4731(88)90159-8

PMID:3260306

Abstract

Androgen receptors were partially purified by affinity chromatography of cytosols prepared from cultured genital fibroblasts of normal individuals and patients with androgen receptor abnormalities. Partially purified receptors were covalently labelled with [3H]R1881 (triated methyltrienolone) by ultraviolet photoactivation. Gel electrophoresis of cytosols from normal individuals showed a single radioactive peak, Mr approximately 97 K. Cytosols from patients with decreased nuclear transfer showed a similar peak, Mr approximately 97 K; cytosols from patients with partial androgen insensitivity (PAIS) or receptor positive complete androgen insensitivity (CAIS, R+) showed a peak Mr approximately 30 K-43 K.

摘要

通过对正常个体以及雄激素受体异常患者的培养生殖成纤维细胞制备的胞质溶胶进行亲和层析，对雄激素受体进行了部分纯化。通过紫外线光活化，将部分纯化的受体与[3H]R1881（氚化甲基三烯olone）进行共价标记。正常个体胞质溶胶的凝胶电泳显示出一个单一的放射性峰，分子量约为97K。核转运减少患者的胞质溶胶显示出类似的峰，分子量约为97K；部分雄激素不敏感（PAIS）或受体阳性完全雄激素不敏感（CAIS，R+）患者的胞质溶胶显示出一个分子量约为30K - 43K的峰。