Thalassemia and Hemoglobinopathy Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran.
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Recent Pat Anticancer Drug Discov. 2020;15(2):174-184. doi: 10.2174/1574892815666200630102944.
A large number of Euphorbia species have been evaluated for anticancer effects; however, their anticancer mechanisms have not been established up to now.
The present study aimed to evaluate the effects of Euphorbia microsciadia (E. microsciadia) Boiss on the modulation of micro (mi) RNAs in MDA-MB-231 cell line.
As the first step, the inhibitory concentration of hydroalcoholic extract of E. microsciadia on MDA-MB-231 cells was examined using the MTT assay, bypassing 24 and 48h from seeding. The real-time quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) was also utilized to determine Let-7, miR-15, miR-16, miR-29, miR-151, miR-155, miR-21, miR-146b, miR-181b, miR-221, miR-222, miR-21, and miR-146b expressions in MDA-MB-231 cells, by passing 24 and 48h from treating with the extract of E. microsciadia.
The results reveal the cytotoxic effects of E. microsciadia on MDA-MB-231 cell line in a dose-dependent manner. The half maximal Inhibitory Concentrations (IC50) were also equal to 275 and 240μg/ml for E. microsciadia, by passing 24 and 48h from the treatment, respectively. Furthermore, it was confirmed that, E. microsciadia had augmented the expression levels of Let-7, miR-15, miR-16, miR-29, and miR-34a, which lead to an increase in apoptosis.
E. microsciadia could modulate some miRNAs involved in cell cycle arrest and apoptosis in MDA-MB-231 cell line. Accordingly, targeting miRNAs by E. microsciadia can open some newer avenues for breast cancer therapy.
大量的大戟属植物已被评估具有抗癌作用，但至今其抗癌机制尚未建立。
本研究旨在评估大戟 Microsciadia（E. microsciadia）Boiss 对 MDA-MB-231 细胞系中 micro（mi）RNAs 调节的影响。
作为第一步，通过在接种后 24 和 48 小时避开 MTT 测定法检查大戟 Microsciadia 水醇提取物对 MDA-MB-231 细胞的抑制浓度。还利用实时定量逆转录聚合酶链反应（qRT-PCR）来确定 Let-7、miR-15、miR-16、miR-29、miR-151、miR-155、miR-21、miR-146b、miR-181b、miR-221、miR-222、miR-21 和 miR-146b 在 MDA-MB-231 细胞中的表达，通过在提取物处理 MDA-MB-231 细胞 24 和 48 小时后 passing。
结果显示，E. microsciadia 以剂量依赖性方式对 MDA-MB-231 细胞系具有细胞毒性作用。半最大抑制浓度（IC50）也分别等于 275 和 240μg/ml，用于 E. microsciadia，通过在处理后 24 和 48 小时 passing。此外，证实 E. microsciadia 增加了 Let-7、miR-15、miR-16、miR-29 和 miR-34a 的表达水平，从而导致细胞凋亡增加。
E. microsciadia 可以调节 MDA-MB-231 细胞系中参与细胞周期停滞和细胞凋亡的一些 miRNAs。因此，E. microsciadia 通过靶向 miRNAs 为乳腺癌治疗开辟了新途径。
