IEEE Trans Nanobioscience. 2020 Jul;19(3):477-484. doi: 10.1109/TNB.2020.2998520.
In this work, a photosensitive (PS) optical fiber-based Mach-Zehnder interferometer (MZI) structure is developed to diagnose the presence of collagen-IV in human bodies. The MZI is fabricated by sequentially splicing the single mode-multimode-photosensitive-multimode-single mode (SMPMS) fiber segments. The sensing region in MZI structure is created by partially removing the cladding of photosensitive fiber by using 40% hydrofluoric (HF) acid and depositing the layers of highly reflective metal nanoparticles (NPs) over it. The used NPs are polyvinyl alcohol stabilized silver nanoparticles (PVA-AgNPs), gold nanoparticles (AuNPs), and zinc oxide nanoparticles (ZnO-NPs). The size of AuNPs, PVA-AgNPs, and ZnO-NPs are 10 ± 0.2 nm, ∼ 4 -5 nm, and < 50 nm, respectively. In order to avoid the interference of other biomolecules in the detection of collagen-IV, the sensing region is functionalized with a collagenase enzyme. The sensing ability of the probe is ascertained by sensing a wide concentration of collagen solution ranging from 0 ng/ml to [Formula: see text]/ml. It is observed that sensing performance of probe is much better on immobilizing it with PVA-AgNPs and ZnO-NPs.
在这项工作中，开发了一种基于光敏光纤的马赫-曾德尔干涉仪（MZI）结构，用于诊断人体中胶原蛋白-IV 的存在。MZI 通过顺序拼接单模-多模-光敏-多模-单模（SMPMS）光纤段来制造。MZI 结构中的传感区通过使用 40%氢氟酸（HF）部分去除光敏光纤的包层并在其上沉积高度反射的金属纳米粒子（NPs）层来创建。所用的 NPs 是聚乙烯醇稳定的银纳米粒子（PVA-AgNPs）、金纳米粒子（AuNPs）和氧化锌纳米粒子（ZnO-NPs）。AuNPs、PVA-AgNPs 和 ZnO-NPs 的尺寸分别为 10 ± 0.2 nm、∼4-5 nm 和 <50 nm。为了避免在检测胶原蛋白-IV 时其他生物分子的干扰，传感区用胶原酶进行了功能化。通过检测从 0 ng/ml 到[公式：见文本]/ml 的宽浓度胶原蛋白溶液来确定探针的传感能力。观察到，探针在固定在 PVA-AgNPs 和 ZnO-NPs 上时的传感性能要好得多。
