University of Applied Sciences Upper Austria, Linz, Austria.
Johannes Kepler University Linz, Linz, Austria.
PLoS Comput Biol. 2020 Jun 30;16(6):e1007902. doi: 10.1371/journal.pcbi.1007902. eCollection 2020 Jun.
We present the software platform 2CALM that allows for a comparative analysis of 3D localisation microscopy data representing protein distributions in two biological samples. The in-depth statistical analysis reveals differences between samples at the nanoscopic level using parameters such as cluster-density and -curvature. An automatic classification system combines multiplex and multi-level statistical approaches into one comprehensive parameter for similarity testing of the compared samples. We demonstrated the biological importance of 2CALM, comparing the protein distributions of CD41 and CD62p on activated platelets in a 3D artificial clot. Additionally, using 2CALM, we quantified the impact of the inflammatory cytokine interleukin-1β on platelet activation in clots. The platform is applicable to any other cell type and biological system and can provide new insights into biological and medical applications.
我们介绍了软件平台 2CALM，它允许对代表两个生物样本中蛋白质分布的 3D 定位显微镜数据进行比较分析。深入的统计分析使用簇密度和曲率等参数在纳米级水平上揭示了样品之间的差异。自动分类系统将多重和多层次统计方法结合到一个综合参数中，用于比较样品的相似性测试。我们通过比较 3D 人工凝块中活化血小板上的 CD41 和 CD62p 的蛋白质分布，证明了 2CALM 的生物学重要性。此外，我们使用 2CALM 量化了炎症细胞因子白细胞介素 1β 对凝块中血小板活化的影响。该平台适用于任何其他细胞类型和生物系统，并可为生物学和医学应用提供新的见解。
