Department of Restorative Dentistry, Sutcu Imam University, Kahramanmaras, Turkey.
Department of Medical Biology and Genetics, Recep Tayyip Erdogan University, Rize, Turkey.
J Oral Biosci. 2020 Sep;62(3):272-279. doi: 10.1016/j.job.2020.06.004. Epub 2020 Jun 27.
Beta-defensin 1, encoded by the DEFB1 gene, is an important molecule that confers protection from dental caries. Numerous studies have been conducted on the rs11362 polymorphism in the DEFB1 gene. We evaluated the results from studies that have investigated the association between rs11362 polymorphism and dental caries, through a meta-analysis.
This meta-analysis was designed according to the PRISMA statement guideline. Electronic databases (PubMed, Web of Science, Scopus, and Cochrane Library) were scanned by two independent researchers. The publication bias was determined by statistical analyses using funnel plot, Egger regression test, and Begg and Mazumdar rank correlation test. Heterogeneity was evaluated using the chi-square test, tau-square, and Higgins I test. Odds ratio (OR) was used to measure the effect size.
Rank correlation and regression procedures showed the absence of publication bias in the meta-analysis (p > 0.05). The DEFB1 rs11362 polymorphism in the heterozygous (CC vs. CT: OR = 2.20, 95% confidence interval (CI): 1.17, 4.10; p = 0.014) and dominant (CC vs. CT + TT: OR = 3.11, 95% CI: 1.18, 8.21; p = 0.022) models in the permanent dentition subgroup showed significant differences. However, there was no significant difference between any model in either the deciduous dentition (p > 0.05) or the mixed dentition subgroups (p > 0.05).
This meta-analysis suggests that the DEFB1 rs11362 polymorphism is associated with dental caries in permanent dentition. Moreover, individuals with the TT genotype were found to have seven times higher risk of dental caries than individuals with the CC genotype. There was no such association or statistical difference observed for deciduous and mixed dentitions.
β-防御素 1 由 DEFB1 基因编码，是一种对抗龋齿的重要分子。已有大量研究针对 DEFB1 基因中的 rs11362 多态性展开。本研究通过荟萃分析，对探讨 rs11362 多态性与龋齿相关性的研究结果进行评估。
本荟萃分析按照 PRISMA 声明指南设计。两位独立研究者通过电子数据库（PubMed、Web of Science、Scopus 和 Cochrane Library）进行检索。通过漏斗图、Egger 回归检验、Begg 和 Mazumdar 等级相关检验进行统计学分析，评估发表偏倚。采用卡方检验、tau 平方检验和 Higgins I 检验评估异质性。采用比值比（OR）衡量效应大小。
秩相关和回归分析表明，荟萃分析中不存在发表偏倚（p＞0.05）。在恒牙亚组中，杂合子（CC 与 CT：OR=2.20，95%置信区间（CI）：1.174.10；p=0.014）和显性模型（CC 与 CT+TT：OR=3.11，95%CI：1.188.21；p=0.022）中，DEFB1 rs11362 多态性存在显著差异。然而，在乳牙（p＞0.05）或混合牙列亚组（p＞0.05）中，任何模型之间均无显著差异。
本荟萃分析表明，DEFB1 rs11362 多态性与恒牙龋齿有关。此外，与 CC 基因型个体相比，TT 基因型个体的龋齿风险高 7 倍。乳牙和混合牙列未观察到这种关联或统计学差异。
