Hatipoğlu Ömer, Saydam Faruk
Department of Restorative Dentistry, Recep Tayyip Erdogan University, Rize, Turkey.
Department of Medical Biology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey.
BMC Oral Health. 2025 May 14;25(1):722. doi: 10.1186/s12903-025-06074-3.
Dental caries is a multifactorial disease influenced by environmental, behavioral, and genetic factors. Recent studies suggest that variations in the AMY1 gene, which encodes salivary amylase, may contribute to caries susceptibility. This study investigates the relationship between AMY1 gene copy number variation (CNV) and dental caries in a Turkish population.
A total of 154 participants (63% female; mean age 19.6 ± 1.4 years) were included. Epithelial cells in inner cheek tissues were collected from volunteers using swabs, and the collected samples were preserved and stored in a DNA stabilization solution. The demographic characteristics of the volunteers were recorded, and DMFT and DMFS index scores were documented on the provided forms. The AMY1 gene CNVs were determined using a Real-time polymerase reaction device. The TaqMan chemistry, which comprises quantitative real-time PCR reactions utilizing a dual TaqMan kit, was utilized in this analysis process. Statistical analyses included the Kruskal-Wallis and Mann-Whitney U tests for group comparisons, Spearman's correlation analysis, and binomial logistic regression to evaluate associations between AMY1 CNVs and dental caries indices.
No statistically significant differences were observed between AMY1 CNVs and DMFT or DMFS indices (p > 0.05). However, significant associations were found between daily tooth brushing frequency and caries indices (Cramer's V = 0.219, p < 0.05), as well as between preferred beverage consumption and caries indices (Cramer's V = 0.219, p < 0.05). Other factors, including gender, dental floss and mouthwash use, and tongue brushing, did not show statistically significant associations (p > 0.05).
Although AMY1 CNVs were not significantly associated with caries indices, the findings highlight the complex interplay of genetic, behavioral, and dietary factors in caries development. This study emphasizes the critical role of oral hygiene and dietary habits in caries prevention and underscores the need for further research on genetic contributions to oral health.
Not applicable.
龋齿是一种受环境、行为和遗传因素影响的多因素疾病。最近的研究表明，编码唾液淀粉酶的AMY1基因的变异可能与龋齿易感性有关。本研究调查了土耳其人群中AMY1基因拷贝数变异（CNV）与龋齿之间的关系。
共纳入154名参与者（63%为女性；平均年龄19.6±1.4岁）。使用棉签从志愿者的内侧脸颊组织中收集上皮细胞，并将收集的样本保存在DNA稳定溶液中。记录志愿者的人口统计学特征，并在提供的表格上记录DMFT和DMFS指数得分。使用实时聚合酶反应装置确定AMY1基因CNV。在该分析过程中使用了TaqMan化学方法，该方法包括利用双TaqMan试剂盒的定量实时PCR反应。统计分析包括用于组间比较的Kruskal-Wallis和Mann-Whitney U检验、Spearman相关性分析以及二项逻辑回归，以评估AMY1 CNV与龋齿指数之间的关联。
在AMY1 CNV与DMFT或DMFS指数之间未观察到统计学上的显著差异（p>0.05）。然而，发现每日刷牙频率与龋齿指数之间存在显著关联（克莱默V=0.219，p<0.05），以及偏好的饮料消费与龋齿指数之间存在显著关联（克莱默V=0.219，p<0.05）。其他因素，包括性别、使用牙线和漱口水以及刷舌，未显示出统计学上的显著关联（p>0.05）。
虽然AMY1 CNV与龋齿指数没有显著关联，但研究结果突出了遗传、行为和饮食因素在龋齿发展中的复杂相互作用。本研究强调了口腔卫生和饮食习惯在预防龋齿中的关键作用，并强调需要进一步研究基因对口腔健康的贡献。
不适用。
