Department of Biochemistry, College of Health Sciences, Addis Ababa University, Ethiopia.
Department of Obstetrics and Gynecology, Institute of Clinical Sciences Lund, Lund University, Sweden.
J Reprod Immunol. 2020 Sep;141:103169. doi: 10.1016/j.jri.2020.103169. Epub 2020 Jun 20.
Preeclampsia (PE) is a human specific pregnancy-related syndrome of unknown etiology that affects 2-8 % of pregnancies. Polymorphism in maternal Killer Cell Immunoglobulin-like Receptors (KIRs) and the ligand fetal Human Leukocyte Antigen-C (HLA-C) may predispose pregnant mothers for PE due to defective trophoblast invasion into the maternal decidua. Our study aimed to investigate the association between maternal KIR and fetal HLA-C polymorphism and PE in Ethiopian pregnant women.
We included a total of 288 (157 controls and 131 PE cases) in a case-controls study at Adama Regional Referral Hospital, Ethiopia. The KIR and HLA-C genotyping was done using traditional polymerase chain reaction on genomic DNA extracted form maternal venous and cord blood followed by 2% agarose gel electrophoresis.
The statistical associations between variables were evaluated using Pearson's Chi-square test. P < 0.05, with 95 % confidence interval was considered statistically significant. A significant association was observed between the KIR2DS1 and PE, with a higher frequency (60.5 %) of the gene in the control group. Similarly, a significant association was observed between KIR AA genotype and PE, with a higher frequency (38.2 %) of this genotype in the PE group. Ethiopians share the same risk genotype for PE as seen in previous African and European studies, namely homozygosity of a maternal KIR AA genotype. However, Ethiopians differ from other East African populations by sharing the same protective KIR2DS1 gene as Europeans.
子痫前期（PE）是一种病因不明的人类特有的妊娠相关综合征，影响 2-8%的妊娠。母体杀伤细胞免疫球蛋白样受体（KIR）和配体胎儿人类白细胞抗原-C（HLA-C）的多态性可能导致妊娠母亲发生 PE，因为胎盘滋养细胞侵入母体蜕膜存在缺陷。我们的研究旨在调查埃塞俄比亚孕妇母体 KIR 和胎儿 HLA-C 多态性与 PE 之间的关联。
我们在埃塞俄比亚阿达玛地区转诊医院进行了一项病例对照研究，共纳入 288 名孕妇（157 名对照组和 131 名 PE 病例）。使用传统聚合酶链反应（PCR）对从母体静脉和脐带血中提取的基因组 DNA 进行 KIR 和 HLA-C 基因分型，然后进行 2%琼脂糖凝胶电泳。
使用 Pearson 卡方检验评估变量之间的统计学关联。P 值<0.05，95%置信区间被认为具有统计学意义。KIR2DS1 与 PE 之间存在显著关联，在对照组中该基因的频率较高（60.5%）。同样，KIR AA 基因型与 PE 之间存在显著关联，在 PE 组中该基因型的频率较高（38.2%）。埃塞俄比亚人与之前的非洲和欧洲研究一样，具有相同的 PE 风险基因型，即母体 KIR AA 基因型的纯合性。然而，埃塞俄比亚人与其他东非人群不同，因为他们与欧洲人一样具有相同的保护性 KIR2DS1 基因。
