School of Integrative Engineering, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul, 06974, South Korea.
J Nanosci Nanotechnol. 2020 Nov 1;20(11):7163-7168. doi: 10.1166/jnn.2020.18853.
A label-free electrochemical impedimetric immunosensor for the detection of Triiodothyronine-a thyroid hormone that functions as the biomarker for monitoring for thyroid dysfunction was developed. The gold nanoparticle-modified electrode was employed to achieve the sensitive determination of Triiodothyronine at a low concentration level. The gold nanoparticle layer on the gold electrode was generated by chronoamperometry method and its resulting characteristics were investigated by scanning electron microscopy. Redox probe [Fe(CN)] and electrochemical impedance spec-troscopy was used for both evaluation of the immobilization of anti-Triiodothyronine antibody on the electrode surface and quantitative determination of target Triiodothyronine in different concentrations. The electrode with absorbed antibodies showed significant changes in charge transfer resistance upon binding the antigen, which resulted in an increase in normalized impedance change as the addition of antigen concentrations over a dynamic linear range of 0.01-100 ng/ml. These results indicated that the proposed immunosensor could be a potential alternative method for determination of Triiodothyronine in clinics with the advantage of low cost and less time-consuming.
一种用于检测三碘甲状腺原氨酸（甲状腺激素的一种，作为监测甲状腺功能障碍的生物标志物）的无标记电化学阻抗免疫传感器被开发出来。金纳米粒子修饰电极用于在低浓度水平下实现对三碘甲状腺原氨酸的灵敏测定。金电极上的金纳米粒子层通过计时安培法生成，并通过扫描电子显微镜研究其所得特性。氧化还原探针[Fe(CN)]和电化学阻抗谱用于评估抗三碘甲状腺原氨酸抗体在电极表面的固定化以及不同浓度下目标三碘甲状腺原氨酸的定量测定。在加入抗原后，吸附有抗体的电极的电荷转移电阻发生显著变化，导致归一化阻抗变化随着抗原浓度的增加而增加，在 0.01-100ng/ml 的动态线性范围内。这些结果表明，该免疫传感器可作为一种潜在的替代方法，用于临床中测定三碘甲状腺原氨酸，具有成本低、耗时少的优点。
