Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.
Food4Future Technologies Sp. z o.o., ul. Tarasa Szewczenki 24, 51-351 Wrocław, Poland.
Sensors (Basel). 2023 Jan 5;23(2):602. doi: 10.3390/s23020602.
This article presents a novel and selective electrochemical bioassay with antibody and laccase for the determination of free thyroid hormone (free triiodothyronine, fT3). The biosensor was based on a glassy carbon electrode modified with a FeO@graphene nanocomposite with semiconducting properties, an antibody (anti-PDIA3) with high affinity for fT3, and laccase, which was responsible for catalyzing the redox reaction of fT3. The electrode modification procedure was investigated using a cyclic voltammetry technique, based on the response of the peak current after modifications. All characteristic working parameters of the developed biosensor were analyzed using differential pulse voltammetry. Obtained experimental results showed that the biosensor revealed a sensitive response to fT3 in a concentration range of 10-200 µM, a detection limit equal to 27 nM, and a limit of quantification equal to 45.9 nM. Additionally, the constructed biosensor was selective towards fT3, even in the presence of interference substances: ascorbic acid, tyrosine, and levothyroxine, and was applied for the analysis of fT3 in synthetic serum samples with excellent recovery results. The designed biosensor also exhibited good stability and can find application in future medical diagnostics.
本文提出了一种新颖而选择性的电化学生物测定方法，用于测定游离甲状腺激素（游离三碘甲状腺原氨酸，fT3）。该生物传感器基于具有半导体性质的 FeO@graphene 纳米复合材料修饰的玻碳电极、对 fT3 具有高亲和力的抗体（抗-PDIA3）和漆酶，漆酶负责催化 fT3 的氧化还原反应。通过循环伏安技术研究了电极修饰过程，基于修饰后的峰电流响应。使用差分脉冲伏安法分析了所开发的生物传感器的所有特征工作参数。获得的实验结果表明，该生物传感器对 fT3 在 10-200 µM 的浓度范围内表现出灵敏的响应，检测限等于 27 nM，定量限等于 45.9 nM。此外，该构建的生物传感器对 fT3 具有选择性，即使存在干扰物质：抗坏血酸、酪氨酸和左甲状腺素，并且可用于分析合成血清样品中的 fT3，具有出色的回收率结果。设计的生物传感器还表现出良好的稳定性，可在未来的医学诊断中得到应用。
