Department of Animal Science, Universidade Federal de Roraima-UFRR, BR 174, km 12, 69300-000 Boa Vista, Roraima, Brazil.
Department of Animal Science, Universidade de São Paulo-USP, Av. Duque de Caxias Norte, 225, 13635-900 Pirassununga, São Paulo, Brazil.
Molecules. 2020 Jun 26;25(12):2943. doi: 10.3390/molecules25122943.
Condensed tannins (CTs) are plant anti-herbivore compounds with antimicrobial activity that can be used in ruminant diets as ruminal microbiome manipulators. However, not all CTs from fodder legumes are bioactive due to their wide structural diversity. The aim of our study was to investigate the effect of 10 CT-containing plants , , , , , , , , and ) on in vitro fermentation kinetics of Nelore beef cattle. Polyethylene glycol (PEG), a specific CT-binding agent, was added to neutralize condensed tannin. Tifton and alfalfa hay were used as controls lacking CT. The experimental layout included a randomized complete block with factorial design and four blocks. The data were subjected to analysis of variance followed by Duncan's test to determine differences (p < 0.05) among treatment means. The addition of PEG in browse incubations resulted in increased gas production, fermentation rate, short-chain fatty acid (SCFA) and N-NH release. Within our study, , , , , and showed superior bioactivity compared to other species evaluated, suggesting a natural alternative for replacing ionophores to modify ruminal fermentation. Condensed tannins from , , , , and have the potential to modify rumen fermentation in beef cattle.
缩合单宁（CTs）是具有抗食草动物活性的植物抗草化合物，可作为瘤胃微生物组操纵剂用于反刍动物日粮。然而，由于其广泛的结构多样性，并非所有饲料豆类中的 CT 都具有生物活性。我们研究的目的是研究 10 种含有 CT 的植物，包括、、、、、、、和）对巴西内罗尔肉牛体外发酵动力学的影响。聚乙二醇（PEG）是一种特定的 CT 结合剂，用于中和缩合单宁。蒂夫顿干草和紫花苜蓿干草用作缺乏 CT 的对照。实验设计包括随机完全区组和析因设计，共 4 个区组。数据采用方差分析，然后用邓肯氏检验确定处理均值之间的差异（p<0.05）。在浏览孵育中添加 PEG 会增加气体产生、发酵速率、短链脂肪酸（SCFA）和 N-NH 释放。在我们的研究中，与其他评估的物种相比，、、、和表现出更高的生物活性，这表明可以替代离子载体来改变瘤胃发酵的天然替代品。来自、、、和的缩合单宁有可能改变肉牛的瘤胃发酵。
