Turner M, Feldmann M
Charing Cross Sunley Research Centre, Hammersmith, London.
Biochem Biophys Res Commun. 1988 Jun 30;153(3):1144-51. doi: 10.1016/s0006-291x(88)81347-0.
The expression of the mRNA encoding tumour necrosis factor, lymphotoxin and interleukin-6 by peripheral blood mononuclear cells was analysed. Unstimulated cells contained no detectable mRNA for these cytokines, however each mRNA was transiently expressed after stimulation with either the combination of phytohaemagglutinin and phorbol ester or the single stimulus of lipopolysaccharide. The dual stimulus yielded the stronger signal. The cytokine mRNA's had short half lives, but were stabilised following protein synthesis inhibition. Cyclosporin A completely blocked induction of lymphotoxin and partially inhibited induction of TNF and IL-6 mRNA. The features of regulation described in this paper suggest these genes belong within the "early" set of genes expressed following immune cell activation.
分析了外周血单个核细胞中编码肿瘤坏死因子、淋巴毒素和白细胞介素-6的mRNA的表达情况。未受刺激的细胞中未检测到这些细胞因子的mRNA，但在用植物血凝素和佛波酯组合刺激或脂多糖单一刺激后，每种mRNA均短暂表达。双重刺激产生更强的信号。细胞因子mRNA的半衰期较短，但在蛋白质合成抑制后得以稳定。环孢素A完全阻断淋巴毒素的诱导，并部分抑制TNF和IL-6 mRNA的诱导。本文所述的调控特征表明，这些基因属于免疫细胞激活后表达的“早期”基因集合。
