Grubeck-Loebenstein B, Buchan G, Chantry D, Kassal H, Londei M, Pirich K, Barrett K, Turner M, Waldhausl W, Feldmann M
Charing Cross Sunley Research Centre, London, England.
Clin Exp Immunol. 1989 Sep;77(3):324-30.
Cytokine production was studied in thyroid tissue from patients with Graves' disease, Hashimoto's thyroiditis and non-toxic goitre. The expression of interferon gamma, tumour necrosis factor alpha and beta, interleukin-1 alpha and beta, interleukin-6 and platelet-derived growth factor A chain was assessed by slot-blot analysis of the respective mRNA in freshly isolated tissue samples. All seven cytokines were detected in patients of all groups. Although the respective mRNA levels were, in general, higher in thyroid autoimmune disorders, this appeared to relate to the degree of the lymphocytic infiltration of the thyroid gland at the time of surgery. Purified thyroid follicular cells expressed high levels of interleukin-1 alpha and interleukin-6 mRNA and when established in primary culture, purified thyroid follicular cells from Graves' disease as well as non-toxic goitre produced interleukin-1 alpha and interleukin-6 bioactivity spontaneously. In the case of interleukin-1 this could be further augmented by addition of lipopolysaccharide to the thyroid follicular cell cultures. These results demonstrate that the lymphocytic infiltrate found in autoimmune and non-autoimmune thyroid disorders is associated with cytokine production. Additionally we have shown that intrathyroidal cytokine production is not restricted to thyroid-infiltrating mononuclear cells, but may also involve thyroid follicular cells both in vivo and in vitro. The cytokines produced by thyroid follicular cells may have an important role in stimulating autoantigen specific T cells in vivo as both interleukin-1 and interleukin-6 facilitate T cell activation.
在格雷夫斯病、桥本甲状腺炎和非毒性甲状腺肿患者的甲状腺组织中研究了细胞因子的产生。通过对新鲜分离组织样本中各自mRNA的狭缝印迹分析，评估了干扰素γ、肿瘤坏死因子α和β、白细胞介素-1α和β、白细胞介素-6以及血小板衍生生长因子A链的表达。在所有组的患者中均检测到了所有七种细胞因子。尽管一般来说，甲状腺自身免疫性疾病中各自的mRNA水平较高，但这似乎与手术时甲状腺的淋巴细胞浸润程度有关。纯化的甲状腺滤泡细胞表达高水平的白细胞介素-1α和白细胞介素-6 mRNA，并且在原代培养中，来自格雷夫斯病以及非毒性甲状腺肿的纯化甲状腺滤泡细胞自发产生白细胞介素-1α和白细胞介素-6生物活性。就白细胞介素-1而言，向甲状腺滤泡细胞培养物中添加脂多糖可进一步增强其活性。这些结果表明，在自身免疫性和非自身免疫性甲状腺疾病中发现的淋巴细胞浸润与细胞因子的产生有关。此外，我们已经表明，甲状腺内细胞因子的产生不仅限于甲状腺浸润性单核细胞，而且在体内和体外可能还涉及甲状腺滤泡细胞。甲状腺滤泡细胞产生的细胞因子可能在体内刺激自身抗原特异性T细胞方面发挥重要作用，因为白细胞介素-1和白细胞介素-6都有助于T细胞活化。
